Rabbit anti-Human CRH Polyclonal Antibody | anti-CRH antibody

CRH, CT (CRH, Corticoliberin, Corticotropin-releasing factor, Corticotropin-releasing hormone) (AP)

Cat. Num. AAA6288802

• Gene Names: CRH; CRF
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CRH; Polyclonal Antibody; CT (CRH; Corticoliberin; Corticotropin-releasing factor; Corticotropin-releasing hormone) (AP); Corticotropin-releasing hormone; anti-CRH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CRH antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CRH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-164 amino acids from the C-terminal region of human CRH.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CRH (arrow) using rabbit polyclonal CRH Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CRH gene.)

Related Product Information for anti-CRH antibody

Corticotropin-releasing hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in this protein has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition.

Product Categories/Family for anti-CRH antibody

Antibodies; Hormones; Steroids

References

Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010). Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010): Holliday, K.L., et al. J Psychosom Res 68(5):469-474(2010). Binder, E.B., et al. Arch. Gen. Psychiatry 67(4):369-379(2010). Kageyama, K., et al. Vitam. Horm. 82, 301-317 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4503041
• NCBI GeneID: 1392
• NCBI Accession #: NP_000747.1
• NCBI GenBank Nucleotide #: NM_000756.2
• UniProt Accession #: P06850; B3KQS4
• Molecular Weight: 21,422 Da
• NCBI Official Full Name: corticoliberin preproprotein
• NCBI Official Synonym Full Names: corticotropin releasing hormone
• NCBI Official Symbol: CRH
• NCBI Official Synonym Symbols: CRF
• NCBI Protein Information: corticoliberin; corticotropin-releasing factor
• UniProt Protein Name: Corticoliberin
• Protein Family: Corticoliberin
• UniProt Gene Name: CRH
• UniProt Synonym Gene Names: CRF
• UniProt Entry Name: CRF_HUMAN

Uniprot Description

CRH: This hormone from hypothalamus regulates the release of corticotropin from pituitary gland. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q13

Cellular Component: extracellular space; cytoplasm; extracellular region; perikaryon

Molecular Function: corticotropin-releasing hormone receptor 1 binding; neuropeptide hormone activity; protein binding; hormone activity; corticotropin-releasing hormone receptor 2 binding; adrenocorticotropin-releasing hormone activity; receptor binding

Biological Process: diterpenoid metabolic process; negative regulation of circadian sleep/wake cycle, REM sleep; synaptic transmission, dopaminergic; hypothalamus development; positive regulation of cortisol secretion; induction of apoptosis by hormones; response to pain; female pregnancy; signal transduction; positive regulation of adrenocorticotropic hormone secretion; negative regulation of luteinizing hormone secretion; synaptic transmission; learning and/or memory; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; feeding behavior; parturition; negative regulation of blood pressure; inflammatory response; response to electrical stimulus; associative learning; response to corticosterone stimulus; response to drug; adrenal gland development; response to ether; response to ethanol; long-term memory; ion homeostasis; response to estrogen stimulus; regulation of serotonin secretion; negative regulation of epinephrine secretion; positive regulation of protein amino acid phosphorylation; glucocorticoid biosynthetic process; lung development

Disease: Acth Deficiency, Isolated