Rabbit anti-Human CRFR2D Polyclonal Antibody | anti-CRFR2D antibody

CRFR2D (Corticotropin-releasing factor receptor 2,CRHR2,CRF2R, CRH2R) (Azide free) (HRP)

Cat. Num. AAA6288795

• Gene Names: CRHR2; CRF2; CRFR2; CRF-RB; HM-CRF
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CRFR2D; Polyclonal Antibody; CRFR2D (Corticotropin-releasing factor receptor 2; CRHR2; CRF2R; CRH2R) (Azide free) (HRP); Corticotropin-releasing factor receptor 2; CRH2R; anti-CRFR2D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CRFR2D antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CRFR2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the human CRFR2D.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CRFR2D Antibody western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CRFR2D antibody detected the CRFR2D protein (arrow).)

Immunohistochemistry (IHC)

(CRFR2D Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CRFR2D Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CRFR2D Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CRFR2D antibody

Liaw et al. (1996) [PubMed 8536644] stated that there are 2 G protein-coupled corticotropin-releasing hormone (CRH; MIM 122560) receptors, CRHR1 (MIM 122561) and CRHR2, which they termed the CRF2 receptor. CRH is a 41-amino acid peptide synthesized in the hypothalamus that is the principal neuroregulator of the hypothalamic-pituitary-adrenocortical axis and plays an important role in coordinating the endocrine, autonomic, and behavioral responses to stress and immune challenge.

Product Categories/Family for anti-CRFR2D antibody

Antibodies; Receptors

References

Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010): Gutknecht, E., et al. Eur. J. Pharmacol. 642 (1-3), 1-9 (2010): Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010). Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010): Joubert, B.R., et al. Genome Med 2 (3), 17 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 321117257
• NCBI GeneID: 1395
• NCBI Accession #: NP_001189412.1
• NCBI GenBank Nucleotide #: NM_001202483.1
• UniProt Accession #: B3SXS6; O43461; Q4QRJ4; Q99431; Q13324
• Molecular Weight: 47,688 Da
• NCBI Official Full Name: corticotropin-releasing factor receptor 2 isoform 5
• NCBI Official Synonym Full Names: corticotropin releasing hormone receptor 2
• NCBI Official Symbol: CRHR2
• NCBI Official Synonym Symbols: CRF2; CRFR2; CRF-RB; HM-CRF
• NCBI Protein Information: corticotropin-releasing factor receptor 2; CRH-R2; CRF2 receptor, beta isoform

NCBI Description

The protein encoded by this gene belongs to the G-protein coupled receptor 2 family, and the subfamily of corticotropin releasing hormone receptor. This receptor shows high affinity for corticotropin releasing hormone (CRH), and also binds CRH-related peptides such as urocortin. CRH is synthesized in the hypothalamus, and plays an important role in coordinating the endocrine, autonomic, and behavioral responses to stress and immune challenge. Studies in mice suggest that this receptor maybe involved in mediating cardiovascular homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jan 2011]