Rabbit anti-Human CRFR1 Polyclonal Antibody | anti-CRFR1 antibody

CRFR1 (Q103) (CRHR1, CRFR, CRFR1, CRHR, Corticotropin-releasing factor receptor 1, Corticotropin-releasing hormone receptor 1) (FITC)

Cat. Num. AAA6288772

• Gene Names: CRHR1; CRF1; CRHR; CRF-R; CRFR1; CRF-R1; CRFR-1; CRH-R1; CRHR1L; CRF-R-1; CRH-R-1
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CRFR1; Polyclonal Antibody; CRFR1 (Q103) (CRHR1; CRFR; CRHR; Corticotropin-releasing factor receptor 1; Corticotropin-releasing hormone receptor 1) (FITC); CRHR1; Corticotropin-releasing hormone receptor 1; anti-CRFR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CRFR1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CRFR1 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 88-119 of human CRFR1. Species sequence homology: rat
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) using 034219. This demonstrates that 034219 detected the CRFR1 protein (arrow).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of K562 cells using 034219 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CRFR1 antibody

This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-CRFR1 antibody

Antibodies; Receptors

References

1. Karteris, E., et al. Endocrinology 151(10):4959-4968(2010). 2. Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010). 3. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 4. Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010). 5. Hillhouse, E.W., et al. Endocr. Rev. 27(3):260-286(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 19923245
• NCBI GeneID: 1394
• NCBI Accession #: NP_004373.2; NP_001138618.1
• NCBI GenBank Nucleotide #: NM_004382.4
• UniProt Accession #: P34998; Q13008; Q4QRJ1; Q9UK64; B4DIE9
• Molecular Weight: 28,122 Da
• NCBI Official Full Name: corticotropin-releasing factor receptor 1 isoform 2
• NCBI Official Synonym Full Names: corticotropin releasing hormone receptor 1
• NCBI Official Symbol: CRHR1
• NCBI Official Synonym Symbols: CRF1; CRHR; CRF-R; CRFR1; CRF-R1; CRFR-1; CRH-R1; CRHR1L; CRF-R-1; CRH-R-1
• NCBI Protein Information: corticotropin-releasing factor receptor 1
• UniProt Protein Name: Corticotropin-releasing factor receptor 1
• UniProt Gene Name: CRHR1
• UniProt Synonym Gene Names: CRFR; CRFR1; CRHR; CRF-R-1; CRF-R1; CRFR-1; CRH-R-1; CRH-R1
• UniProt Entry Name: CRFR1_HUMAN

NCBI Description

This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants. Readthrough transcription also exists between this gene and upstream GeneID:401884 (ADP-ribosylation factor 3 pseudogene), and the readthrough transcripts encode isoforms that share similarity with the products of this gene. [provided by RefSeq, Dec 2014]

Uniprot Description

CRF-R1: a G-protein coupled receptor that specifically binds corticotropin-releasing hormone, a potent mediator of endocrine, autonomic, behavioral, and immune responses to stress. Four splice-variant isoforms have been described.

Protein type: Membrane protein, integral; GPCR, family 2; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: multivesicular body; cell soma; apical part of cell; integral to plasma membrane; dendrite; integral to membrane; plasma membrane; trans-Golgi network; vesicle; intrinsic to plasma membrane

Molecular Function: protein binding; corticotropin-releasing hormone binding; corticotropin-releasing hormone receptor activity; protein complex binding; G-protein alpha-subunit binding; corticotrophin-releasing factor receptor activity

Biological Process: fear response; hypothalamus development; hormone-mediated signaling; adrenal gland development; adenylate cyclase activation; positive regulation of mast cell degranulation; female pregnancy; behavioral response to ethanol; memory; general adaptation syndrome, behavioral process; elevation of cytosolic calcium ion concentration; behavioral response to pain; epithelial cell differentiation; neuropeptide signaling pathway; behavioral response to cocaine; adrenocorticotropin hormone secretion; response to hypoxia; negative regulation of epinephrine secretion; visual learning; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); immune response; parturition; response to electrical stimulus