Rabbit anti-Human CRELD1 Polyclonal Antibody | anti-CRELD1 antibody

Anti-CRELD1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8106934

• Gene Names: CRELD1; AVSD2; CIRRIN
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: CRELD1; Polyclonal Antibody; Anti-CRELD1 Antibody; Rabbit Polyclonal; CRELD1 Antibody; Rabbit PAb; Antigen Affinity Purified; cysteine-rich with EGF-like domains 1; Anti-AVSD2 Antibody; Anti-CIRRIN Antibody; anti-CRELD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CRELD1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CRELD1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IHC-P: 1:2500-1:10,000
• Immunogen: Recombinant Human CRELD1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CRELD1 (rh CRELD1). CRELD1 specific IgG was purified by Human CRELD1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human CRELD1 in human kidney with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human CRELD1 in human kidney with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)

Western Blot (WB)

(Anti-E2F6 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:37 kDa)

Related Product Information for anti-CRELD1 antibody

CRELD1 is a transmembrane glycoprotein. Epidermal growth factor(EGF)like domain exists in CRELD1. EGF-like repeats are a class of cysteine-rich domains that mediate interactions between proteins of diverse function. EGF domains are found in proteins that are either completely secreted or have transmembrane regions that tether the protein to the cell surface. CRELD1 contains a 333 amino acid acid (aa) extracellular domain (ECD), two tandem transmembrane segments, and a second ECD of 15 aa. Defects in CRELD1 may cause susceptibility to atrioventricular septal defect type 2 which results in a persistent common atrioventricular canal.

References

Robinson SW, et al. (2003) Missense Mutations in CRELD1 Are Associated with Cardiac Atrioventricular Septal Defects. Am J Hum Genet. 72(4):1047-52.Zatyka M, et al. (2005) Analysis of CRELD1 as a candidate 3p25 atrioventicular septal defect locus (AVSD2). Clin Genet. 67(6):526-8.Stelzl U, et al. (2005) A human protein-protein interaction network: a resource for annotating the proteome. Cell. 122(6):957-68.

NCBI and Uniprot Product Information

• NCBI GI #: 116734855
• NCBI GeneID: 78987
• NCBI Accession #: NP_001070883.1
• NCBI GenBank Nucleotide #: NM_001077415.2
• Molecular Weight: 45,942 Da
• NCBI Official Full Name: cysteine-rich with EGF-like domain protein 1 isoform 2
• NCBI Official Synonym Full Names: cysteine-rich with EGF-like domains 1
• NCBI Official Symbol: CRELD1
• NCBI Official Synonym Symbols: AVSD2; CIRRIN
• NCBI Protein Information: cysteine-rich with EGF-like domain protein 1
• Protein Family: Cysteine-rich with EGF-like domain protein

NCBI Description

This gene encodes a member of a subfamily of epidermal growth factor-related proteins. The encoded protein is characterized by a cysteine-rich with epidermal growth factor-like domain. This protein may function as a cell adhesion molecule. Mutations in this gene are the cause of atrioventricular septal defect. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]