Rabbit anti-Human CQ068 Polyclonal Antibody | anti-CTC1 antibody

CQ068 Antibody (Center)

Cat. Num. AAA9206674

• Gene Names: CTC1; CRMCC; AAF132; AAF-132; C17orf68; tmp494178
• Reactivity: Human
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: CQ068; Polyclonal Antibody; CQ068 Antibody (Center); CST complex subunit CTC1; Conserved telomere maintenance component 1; HBV DNAPTP1-transactivated protein B; CTC1; C17orf68; anti-CTC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CQ068 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 767-795 amino acids from the Central region of human CQ068.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.44 (varies by lot)
• Sequence Positions: 767-795
• Sequence Length: 1217

• Applicable Applications for anti-CTC1 antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000; FC~~1:10~50
• Antigen Type: Synthetic Peptide
• Antigen Source: Human
• Function: Component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere duplex. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. Initially the CST complex has been proposed to protect telomeres from DNA degradation (PubMed:19854130). However, the CST complex has been shown to be involved in several aspects of telomere replication. The CST complex inhibits telomerase and is involved in telomere length homeostasis; it is proposed to bind to newly telomerase-synthesized 3' overhangs and to terminate telomerase action implicating the association with the ACD:POT1 complex thus interfering with its telomerase stimulation activity. The CST complex is also proposed to be involved in fill-in synthesis of the telomeric C-strand probably implicating recruitment and activation of DNA polymerase alpha (PubMed:22763445). The CST complex facilitates recovery from many forms of exogenous DNA damage; seems to be involved in the re-initiation of DNA replication at repaired forks and/or dormant origins (PubMed:25483097). Involved in telomere maintenance (PubMed:19854131, PubMed:22863775). Involved in genome stability (PubMed:22863775). May be in involved in telomeric C-strand fill-in during late S/G2 phase (By similarity).
• Cellular Location: Nucleus. Chromosome, telomere. Note=A transmembrane region is predicted by sequence analysis tools (ESKW, MEMSAT and Phobius); however, given the telomeric localization of the protein, the relevance of the transmembrane region is unsure in vivo (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication. CQ068 also appears to function in a telomere-associated complex with OBFC1 and TEN1.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of CQ068 Antibody (Center) in K562 cell line lysates (35ug/lane). CQ068 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(CQ068 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CTC1 antibody

CQ068 is subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication. CQ068 also appears to function in a telomere-associated complex with OBFC1 and TEN1.

References

Surovtseva, Y.V., et al. Mol. Cell 36(2):207-218(2009)
Miyake, Y., et al. Mol. Cell 36(2):193-206(2009)
Casteel, D.E., et al. J. Biol. Chem. 284(9):5807-5818(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 155029539
• NCBI GeneID: 80169
• NCBI Accession #: NP_079375.3
• NCBI GenBank Nucleotide #: NM_025099.5
• UniProt Accession #: Q2NKJ3; Q1PCD1; Q2TBE3; Q8N3S6; Q9H6L0; B3KR66; C9JEX5
• Molecular Weight: 134609
• NCBI Official Full Name: CST complex subunit CTC1
• NCBI Official Synonym Full Names: CTS telomere maintenance complex component 1
• NCBI Official Symbol: CTC1
• NCBI Official Synonym Symbols: CRMCC; AAF132; AAF-132; C17orf68; tmp494178
• NCBI Protein Information: CST complex subunit CTC1
• UniProt Protein Name: CST complex subunit CTC1
• Protein Family: CST complex
• UniProt Gene Name: CTC1
• UniProt Synonym Gene Names: C17orf68
• UniProt Entry Name: CTC1_HUMAN

NCBI Description

This gene encodes a component of the CST complex. This complex plays an essential role in protecting telomeres from degradation. This protein also forms a heterodimer with the CST complex subunit STN1 to form the enzyme alpha accessory factor. This enzyme regulates DNA replication. Mutations in this gene are the cause of cerebroretinal microangiopathy with calcifications and cysts. Alternate splicing results in both coding and non-coding variants. [provided by RefSeq, Mar 2012]

Uniprot Description

CTC1: Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites. Defects in CTC1 are the cause of cerebroretinal microangiopathy with calcifications and cysts (CRMCC). An autosomal recessive pleiomorphic disorder characterized primarily by intracranial calcifications, leukodystrophy, and brain cysts. Affected individuals manifest a phenotype consistent with Coats plus syndrome or Labrune syndrome. Coats plus syndrome patients have retinal telangiectasia and exudates as well as extraneurologic manifestations, including osteopenia with poor bone healing and a high risk of gastrointestinal bleeding and portal hypertension caused by vasculature ectasias in the stomach, small intestine, and liver. Some individuals with Coats syndrome also have hair, skin, and nail changes, as well as anemia. Patients with Labrune syndrome do not have extraneurologic manifestations. Belongs to the CTC1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA replication; DNA-binding

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: nuclear chromosome, telomeric region; nucleus

Molecular Function: protein binding; single-stranded DNA binding

Biological Process: bone marrow development; spleen development; positive regulation of fibroblast proliferation; thymus development; multicellular organism growth; regulation of G2/M transition of mitotic cell cycle; response to DNA damage stimulus; telomere maintenance; positive regulation of DNA replication

Disease: Cerebroretinal Microangiopathy With Calcifications And Cysts