Rabbit anti-Human CPT1C Polyclonal Antibody | anti-CPT1C antibody

CPT1C, CT (CPT1C, CATL1, Carnitine O-palmitoyltransferase 1, brain isoform, CPT IC, Carnitine O-palmitoyltransferase I, brain isoform, Carnitine palmitoyltransferase 1C) (AP)

Cat. Num. AAA6288494

• Gene Names: CPT1C; CATL1; CPT1P; CPTIC; SPG73; CPT1-B; CPTI-B
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CPT1C; Polyclonal Antibody; CT (CPT1C; CATL1; Carnitine O-palmitoyltransferase 1; brain isoform; CPT IC; Carnitine O-palmitoyltransferase I; Carnitine palmitoyltransferase 1C) (AP); Carnitine palmitoyltransferase 1C; anti-CPT1C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CPT1C antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CPT1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 759-790 amino acids from the C-terminal region of human CPT1C.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CPT1C Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CPT1C antibody detected the CPT1C protein (arrow).)

Flow Cytometry (FC/FACS)

(CPT1C Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CPT1C antibody

The Cpt1 family of proteins are outer mitochondrial membrane proteins that regulate the entry into, and oxidation of fatty acids by, mitochondria. Malonyl-CoA, an intermediate in fatty acid synthesis, has been implicated as a regulatory component of the energy sensing system that feeds into hypothalmic neurons to impart energy homeostasis. Malonyl-CoA levels in the hypothalamus are dynamically regulated by fasting and feeding, altering subsequent feeding behaviour. Cpt1c, the brain-specific carnitine O-palmitoyltransferase 1, is thought to relay information about malonyl-CoA levels in hypothalamic neurons that express orexigenic and anorexigenic neuropeptides that regulate food intake and peripheral energy expenditure. Unlike other Cpt1 proteins, Cpt1c binds Malonyl-CoA but does not catalyse the transfer of the malonyl group from CoA to carnitine.

Product Categories/Family for anti-CPT1C antibody

Antibodies; Enzymes

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Roomets, E., et al. Invest. Ophthalmol. Vis. Sci. 49(4):1660-1664(2008). Sierra, A.Y., et al. J. Biol. Chem. 283(11):6878-6885(2008). Price, N., et al. Genomics 80(4):433-442(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 209862905
• NCBI GeneID: 126129
• NCBI Accession #: NP_001129524.1; NP_689572.1
• NCBI GenBank Nucleotide #: NM_001136052.2
• UniProt Accession #: Q8TCG5; Q5K6N5; Q8N6Q9; Q8NDS6; Q8TE84; A8K0Z8
• Molecular Weight: 81,000 Da
• NCBI Official Full Name: carnitine O-palmitoyltransferase 1, brain isoform isoform 1
• NCBI Official Synonym Full Names: carnitine palmitoyltransferase 1C
• NCBI Official Symbol: CPT1C
• NCBI Official Synonym Symbols: CATL1; CPT1P; CPTIC; SPG73; CPT1-B; CPTI-B
• NCBI Protein Information: carnitine O-palmitoyltransferase 1, brain isoform
• UniProt Protein Name: Carnitine O-palmitoyltransferase 1, brain isoform
• Protein Family: Carnitine O-palmitoyltransferase
• UniProt Gene Name: CPT1C
• UniProt Synonym Gene Names: CATL1; CPT1-B; CPTI-B
• UniProt Entry Name: CPT1C_HUMAN

NCBI Description

This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein regulates the beta-oxidation and transport of long-chain fatty acids into mitochondria, and may play a role in the regulation of feeding behavior and whole-body energy homeostasis. Alternatively spliced transcript variants encoding multiple protein isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

CPT1C: a member of the carnitine/choline acetyltransferase family. The encoded protein regulates the beta-oxidation and transport of long-chain fatty acids into mitochondria, and may play a role in the regulation of feeding behavior and whole-body energy homeostasis. Alternatively spliced transcript variants encoding multiple protein isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Protein type: Lipid Metabolism - fatty acid; Membrane protein, multi-pass; Membrane protein, integral; Transferase; EC 2.3.1.21

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: endoplasmic reticulum membrane; mitochondrial outer membrane; synapse; cell junction

Molecular Function: carnitine O-palmitoyltransferase activity

Biological Process: fatty acid beta-oxidation; carnitine metabolic process

Disease: Spastic Paraplegia 73, Autosomal Dominant