Rabbit anti-Human CPN2 Polyclonal Antibody | anti-CPN2 antibody

CPN2, NT (Carboxypeptidase N Subunit 2, Carboxypeptidase N Polypeptide 2, Carboxypeptidase N 83kD Chain, Carboxypeptidase N Regulatory Subunit, Carboxypeptidase N Large Subunit, ACBP) (PE)

Cat. Num. AAA6467129

• Gene Names: CPN2; ACBP
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CPN2; Polyclonal Antibody; NT (Carboxypeptidase N Subunit 2; Carboxypeptidase N Polypeptide 2; Carboxypeptidase N 83kD Chain; Carboxypeptidase N Regulatory Subunit; Carboxypeptidase N Large Subunit; ACBP) (PE); anti-CPN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CPN2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CPN2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human CPN2 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CPN2 antibody (N-term) in jurkat cell line lysates (35ug/lane). CPN2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(CPN2 Antibody (N-term) (RB18703) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CPN2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CPN2 Antibody (N-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CPN2 antibody

Antibodies; Enzymes; Carboxypeptidase

References

1. Liu, T., Qian, W.J. J. Proteome Res. 4(6), 2070-2080 (2005). 2. Riley, D.A., Tan, F. Genomics 50(1), 105-108 (1998). 3. Skidgel, R.A. Biochem. Biophys. Res. Commun. 154(3), 1323-1329 (1988).

NCBI and Uniprot Product Information

• NCBI GI #: 635172819
• NCBI GeneID: 1370
• NCBI Accession #: NP_001073982
• NCBI GenBank Nucleotide #: NM_001080513
• UniProt Accession #: P22792
• Molecular Weight: 60kDa
• NCBI Official Full Name: carboxypeptidase N subunit 2
• NCBI Official Synonym Full Names: carboxypeptidase N subunit 2
• NCBI Official Symbol: CPN2
• NCBI Official Synonym Symbols: ACBP
• NCBI Protein Information: carboxypeptidase N subunit 2
• UniProt Protein Name: Carboxypeptidase N subunit 2
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPN2
• UniProt Synonym Gene Names: ACBP
• UniProt Entry Name: CPN2_HUMAN

Uniprot Description

CPN2: The 83 kDa subunit binds and stabilizes the catalytic subunit at 37 degrees Celsius and keeps it in circulation. Under some circumstances it may be an allosteric modifier of the catalytic subunit.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: extracellular region

Molecular Function: enzyme regulator activity

Biological Process: protein stabilization; regulation of catalytic activity