Western Blot (WB)
((0. 01ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat CPN10/HSPE1 Polyclonal Antibody | anti-HSPE1 antibody
Goat anti-CPN10/HSPE1 Antibody
Western Blot: Approx 10kDa band observed in lysates of cell line HepG2 (calculated MW of 10.9kDa according to NP_002148.1). Recommended concentration: 0.01-0.03ug/ml.
Western Blot (WB)
((0. 01ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb 2011]
Uniprot Description
HSPE1: Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. Belongs to the GroES chaperonin family.
Protein type: Mitochondrial
Chromosomal Location of Human Ortholog: 2q33.1
Cellular Component: membrane; mitochondrial matrix; mitochondrion
Molecular Function: ATP binding; chaperone binding; metal ion binding; protein binding; unfolded protein binding
Biological Process: caspase activation; chaperone cofactor-dependent protein folding; osteoblast differentiation; protein folding; response to unfolded protein
Research Articles on HSPE1
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Product Notes
The HSPE1 hspe1 (Catalog #AAA423579) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CPN10/HSPE1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's CPN10/HSPE1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 10kDa band observed in lysates of cell line HepG2 (calculated MW of 10.9kDa according to NP_002148.1). Recommended concentration: 0.01-0.03ug/ml. Researchers should empirically determine the suitability of the HSPE1 hspe1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PEKSQGKVLQ AT. It is sometimes possible for the material contained within the vial of "CPN10/HSPE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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