Rabbit anti-Human, Mouse CPN1 Polyclonal Antibody | anti-CPN1 antibody

CPN1, NT (Carboxypeptidase N Catalytic Chain, CPN, Carboxypeptidase N Polypeptide 1, Carboxypeptidase N Small Subunit, Lysine Carboxypeptidase, Arginine Carboxypeptidase, Kininase-1, Serum Carboxypeptidase N, SCPN, Anaphylatoxin Inactivator, Plasma Carbox

Cat. Num. AAA6467112

• Gene Names: CPN1; CPN; SCPN
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CPN1; Polyclonal Antibody; NT (Carboxypeptidase N Catalytic Chain; CPN; Carboxypeptidase N Polypeptide 1; Carboxypeptidase N Small Subunit; Lysine Carboxypeptidase; Arginine Carboxypeptidase; Kininase-1; Serum Carboxypeptidase N; SCPN; Anaphylatoxin Inactivator; Plasma Carbox; anti-CPN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CPN1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CPN1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human CPN1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of CPN1 antibody (N-term) in K562, CEM, HEpG2 cell line lysates and mouse liver tissue lysates (35ug/lane). CPN1(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of CPN1 (arrow) using rabbit polyclonal CPN1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CPN1 gene.)

Product Categories/Family for anti-CPN1 antibody

Antibodies; Enzymes; Carboxypeptidase

References

1. Davis, D.A., Singer, K.E. Blood 105(12), 4561-4568 (2005). 2. Riley, D.A., Tan, F. Genomics 50(1), 105-108 (1998). 3. Hendriks, D., Vingron, M. Biol. Chem. Hoppe-Seyler 374(9), 843-849 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 4503011
• NCBI GeneID: 1369
• NCBI Accession #: NP_001299
• NCBI GenBank Nucleotide #: NM_001308
• UniProt Accession #: P15169
• Molecular Weight: 50kDa
• NCBI Official Full Name: carboxypeptidase N catalytic chain
• NCBI Official Synonym Full Names: carboxypeptidase N subunit 1
• NCBI Official Symbol: CPN1
• NCBI Official Synonym Symbols: CPN; SCPN
• NCBI Protein Information: carboxypeptidase N catalytic chain
• UniProt Protein Name: Carboxypeptidase N catalytic chain
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPN1
• UniProt Synonym Gene Names: ACBP; CPN; SCPN
• UniProt Entry Name: CBPN_HUMAN

NCBI Description

Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits; this gene encodes the catalytic subunit. Mutations in this gene can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

CPN1: Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation. Defects in CPN1 are the cause of carboxypeptidase N deficiency (CPND). Patients affected present some combination of angioedema or chronic urticaria, as well as hay fever or astma, and have also slightly depressed serum carboxy peptidase N, suggestive of autosomal recessive inheritance of this disorder. Belongs to the peptidase M14 family.

Protein type: EC 3.4.17.3; Secreted, signal peptide; Protease; Secreted

Chromosomal Location of Human Ortholog: 10q24.2

Cellular Component: extracellular space

Molecular Function: zinc ion binding; metallocarboxypeptidase activity

Biological Process: response to glucocorticoid stimulus; proteolysis

Disease: Carboxypeptidase N Deficiency