Hexokinase-3 / HK3 Recombinant Protein | HK3 recombinant protein

Recombinant Human Hexokinase-3 / HK3 Protein (His & GST tag)

Cat. Num. AAA2545076

• Gene Names: HK3; HXK3; HKIII
• Purity: > 93 % as determined by SDS-PAGE
• Synonyms: Hexokinase-3 / HK3; Recombinant Human Hexokinase-3 / HK3 Protein (His & GST tag); IMP-2; IMP2; VICKZ2; p62; HK3 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 93 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 50mM Tris, 100mM NaCl, pH 7.4
• Sequence Length: 923

• Application Notes: The recombinant human HK3/GST chimera consists of 1160 amino acids and has a calculated molecular mass of 127 kDa. It migrates as an approximately 115 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for HK3 recombinant protein

Background: Hexokinase-3, also known as Hexokinase type III, HKIII and HK3, is a protein which belongs to the hexokinase family. Hexokinase-3 / HK3 is an enzyme which in humans is encoded by the HK2 gene. Hexokinases phosphorylate glucose to produce glucose 6-phosphate, committing glucose to the glycolytic pathway. In mammalian tissues hexokinase exists as four isoenzymes encoded by distinct genes. These proteins are homologous and are organized in two homologous domains, with the exception of hexokinase type IV which has only one. This organization is believed to be the result of a duplication and tandem fusion event involving the gene encoding for the ancestral hexokinase. The gene encodes hexokinase-3. Similar to hexokinases-1 and hexokinases-2, this allosteric enzyme is inhibited by its product glucose 6-phosphate.

Description: A DNA sequence encoding the human HK3 (P52790) (Met 1-Val 923) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 194097330
• NCBI GeneID: 3101
• NCBI Accession #: NP_002106.2
• NCBI GenBank Nucleotide #: NM_002115.2
• UniProt Accession #: P52790; Q8N1E7
• Molecular Weight: 99,025 Da
• NCBI Official Full Name: hexokinase-3
• NCBI Official Synonym Full Names: hexokinase 3
• NCBI Official Symbol: HK3
• NCBI Official Synonym Symbols: HXK3; HKIII
• NCBI Protein Information: hexokinase-3
• UniProt Protein Name: Hexokinase-3
• Protein Family: Probable histidine kinase
• UniProt Gene Name: HK3
• UniProt Synonym Gene Names: HK III

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq, Apr 2009]

Uniprot Description

HK3: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - starch and sucrose; EC 2.7.1.1; Kinase, other; Transferase

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: cytosol

Molecular Function: fructokinase activity; glucokinase activity; hexokinase activity; mannokinase activity

Biological Process: cell glucose homeostasis; glucose transport; glycolysis