Rabbit anti-Human CPM Polyclonal Antibody | anti-CPM antibody

CPM, ID (CPM, Carboxypeptidase M) (MaxLight 650)

Cat. Num. AAA6288304

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CPM; Polyclonal Antibody; ID (CPM; Carboxypeptidase M) (MaxLight 650); Carboxypeptidase M; anti-CPM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CPM.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-CPM antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 212-241 in the central region of human CPM, UniProt Accession #NP_938079.1; NP_001865.1; NP_001005502.1; P14384.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of lysate from human kidney tissue lysate, using MBS646835 (1:1000).. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

Western Blot (WB)

(Western Blot analysis of lysates from U-251 MG, 293T cell line (from left to right) (35ug/lane), using MBS646835 (1:1000/lane). A goat anti-rabbit IgG H&L(HRP) (1:5000) was used as the secondary antibody.)

Related Product Information for anti-CPM antibody

The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq].

Product Categories/Family for anti-CPM antibody

Antibodies; Enzymes; Carboxypeptidase

References

1. Rietschel, M., et al. Biol. Psychiatry 68(6):578-585 (2010). 2. Erickson-Johnson, M.R., et al. Mod. Pathol. 22(12):1541-1547 (2009). 3. Marquez-Curtis, L., et al. Stem Cells 26(5):1211-1220 (2008). 4. Zhang, X., et al. J. Biol. Chem. 283(12):7994-8004 (2008). 5. Elortza, F., et al. J. Proteome Res. 5(4):935-943 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 38327526
• NCBI GeneID: 1368
• NCBI Accession #: NP_938079.1; NP_001865.1; NP_001005502.1
• NCBI GenBank Nucleotide #: NM_198320.3
• UniProt Accession #: P14384; Q9H2K9; B2R800
• Molecular Weight: 50,514 Da
• NCBI Official Full Name: carboxypeptidase M
• NCBI Official Synonym Full Names: carboxypeptidase M
• NCBI Official Symbol: CPM
• NCBI Protein Information: carboxypeptidase M
• UniProt Protein Name: Carboxypeptidase M
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPM
• UniProt Synonym Gene Names: CPM
• UniProt Entry Name: CBPM_HUMAN

NCBI Description

The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CPM: Specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins. It is believed to play important roles in the control of peptide hormone and growth factor activity at the cell surface, and in the membrane-localized degradation of extracellular proteins. Belongs to the peptidase M14 family.

Protein type: Protease; Membrane protein, GPI anchor; EC 3.4.17.12

Chromosomal Location of Human Ortholog: 12q14.3

Cellular Component: cell surface; plasma membrane

Molecular Function: zinc ion binding; carboxypeptidase activity; metallocarboxypeptidase activity

Biological Process: anatomical structure morphogenesis; proteolysis