Rabbit CPEB4 Polyclonal Antibody | anti-CPEB4 antibody

CPEB4 Antibody

Cat. Num. AAA9612254

• Gene Names: CPEB4; CPE-BP4; hCPEB-4
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: CPEB4; Polyclonal Antibody; CPEB4 Antibody; CPE binding protein 4; CPE-binding protein 4; CPE-BP4; cpeb4; CPEB4_HUMAN; Cytoplasmic polyadenylation element binding protein 4; Cytoplasmic polyadenylation element-binding protein 4; hCPEB-4; KIAA1673; anti-CPEB4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CPEB4 Antibody detects endogenous levels of total CPEB4.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 729

• Applicable Applications for anti-CPEB4 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IF: 1:100-1:500; ICC: 1:100-1:500; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human CPEB4, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Human Fetal lung, using CPEB4 Antibody. The lane on the left was treated with blocking peptide.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(MBS9612254 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612254) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

Related Product Information for anti-CPEB4 antibody

Function: Sequence-specific RNA-binding protein that binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR (PubMed:24990967). RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism (PubMed:24990967). Regulates activation of unfolded protein response (UPR) in the process of adaptation to ER stress in liver, by maintaining translation of CPE-regulated mRNAs in conditions in which global protein synthesis is inhibited (By similarity). Required for cell cycle progression, specifically for cytokinesis and chromosomal segregation (PubMed:26398195). Plays a role as an oncogene promoting tumor growth and progression by positively regulating translation of t-plasminogen activator/PLAT (PubMed:22138752). Stimulates proliferation of melanocytes (PubMed:27857118). In contrast to CPEB1 and CPEB3, does not play role in synaptic plasticity, learning and memory (By similarity).

Subcellular Location: Cytoplasm. Cell projection>Dendrite. Cell projection>Dendritic spine. Cell junction>Synapse>Postsynaptic density. Cell projection>Axon. Cell projection>Growth cone. Endoplasmic reticulum. Cytoplasm>Perinuclear region.

Tissue Specificity: Expressed in pancreas in islets and ductal cells (at protein level) (PubMed:22138752). Expressed in melanocytes (PubMed:27857118).

Subunit Structure: Interacts with TOB1.

Similarity: The 2 RRM domains and the C-terminal region mediate interaction with CPE-containing RNA (PubMed:24990967). The interdomain linker (564-579) acts as a hinge to fix the relative orientation of the 2 RRMs (PubMed:24990967). The ZZ domain (509-566) coordinates 2 Zn ions and is probably implicated in mediating interactions with other proteins in addition to increasing the affinity of the RRMs for the CPEs (By similarity). Unlike in CPEB1, a continuous polar interface is formed between the 2 RRMs (PubMed:24990967).Belongs to the RRM CPEB family.

NCBI and Uniprot Product Information

• NCBI GI #: 189027090
• NCBI GeneID: 80315
• NCBI Accession #: NP_085130
• NCBI GenBank Nucleotide #: NP_085130.2
• UniProt Accession #: Q17RY0
• Molecular Weight: Observed Molecular Weight: (Observed)80kD.; Predicted Molecular Weight: (Calculated)80kDa.
• NCBI Official Full Name: cytoplasmic polyadenylation element-binding protein 4 isoform a
• NCBI Official Synonym Full Names: cytoplasmic polyadenylation element binding protein 4
• NCBI Official Symbol: CPEB4
• NCBI Official Synonym Symbols: CPE-BP4; hCPEB-4
• NCBI Protein Information: cytoplasmic polyadenylation element-binding protein 4
• UniProt Protein Name: Cytoplasmic polyadenylation element-binding protein 4
• Protein Family: Cytoplasmic polyadenylation element-binding protein
• UniProt Gene Name: CPEB4
• UniProt Synonym Gene Names: KIAA1673; CPE-BP4; CPE-binding protein 4; hCPEB-4
• UniProt Entry Name: CPEB4_HUMAN