Rabbit anti-Human CPEB1 Polyclonal Antibody | anti-CPEB1 antibody

CPEB1, CT (CPEB1, CPEB, Cytoplasmic polyadenylation element-binding protein 1) (APC)

Cat. Num. AAA6288253

• Gene Names: CPEB1; CPEB; CPEB-1; h-CPEB; CPE-BP1; hCPEB-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CPEB1; Polyclonal Antibody; CT (CPEB1; CPEB; Cytoplasmic polyadenylation element-binding protein 1) (APC); Cytoplasmic polyadenylation element-binding protein 1; anti-CPEB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CPEB1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CPEB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 446-475 amino acids from the C-terminal region of human CPEB1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CPEB1 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the CPEB1 antibody detected the CPEB1 protein (arrow).)

Related Product Information for anti-CPEB1 antibody

This gene encodes a member of the cytoplasmic polyadenylation element (CPE) binding protein family. This highly conserved protein binds to a specific RNA sequence called the CPE found in the 3' UTR of some mRNAs. Similar proteins in Xenopus and mouse function to induce cytoplasmic polyadenylation of dormant mRNAs with short polyA tails, resulting in their translation. Members of this protein family regulate translation of cyclin B1 during embryonic cell divisions. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-CPEB1 antibody

Antibodies; Binding Proteins

References

Glahder, J.A., et al. Virus Res. 149(2):217-223(2010). Crowther-Swanepoel, D., et al. Nat. Genet. 42(2):132-136(2010). Hansen, C.N., et al. APMIS 117(1):53-59(2009). Ernoult-Lange, M., et al. Mol. Biol. Cell 20(1):176-187(2009). Burns, D.M., et al. Genes Dev. 22(24):3449-3460(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 119395718
• NCBI GeneID: 64506
• NCBI Accession #: NP_001073001.1; NP_001073002.1
• NCBI GenBank Nucleotide #: NM_001079533.1
• UniProt Accession #: Q9BZB8; Q86W46; Q8IV41; Q9BZB7; Q9H8V5; B7Z6C6
• Molecular Weight: 53,635 Da
• NCBI Official Full Name: cytoplasmic polyadenylation element-binding protein 1 isoform 2
• NCBI Official Synonym Full Names: cytoplasmic polyadenylation element binding protein 1
• NCBI Official Symbol: CPEB1
• NCBI Official Synonym Symbols: CPEB; CPEB-1; h-CPEB; CPE-BP1; hCPEB-1
• NCBI Protein Information: cytoplasmic polyadenylation element-binding protein 1
• UniProt Protein Name: Cytoplasmic polyadenylation element-binding protein 1
• UniProt Gene Name: CPEB1
• UniProt Synonym Gene Names: CPEB; CPE-BP1; CPE-binding protein 1; h-CEBP; hCPEB-1
• UniProt Entry Name: CPEB1_HUMAN

NCBI Description

This gene encodes a member of the cytoplasmic polyadenylation element binding protein family. This highly conserved protein binds to a specific RNA sequence, called the cytoplasmic polyadenylation element, found in the 3' untranslated region of some mRNAs. The encoded protein functions in both the cytoplasm and the nucleus. It is involved in the regulation of mRNA translation, as well as processing of the 3' untranslated region, and may play a role in cell proliferation and tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

CPEB: cytoplasmic polyadenylation element binding protein 1. Binds to the protein CPE and drives its polyadenylation. Activated by the protein kinase AurA. Insulin and progesterone stimulate AurA-catalyzed phosphorylation of CPEB in oocytes. Neuronal CPEB regulates local protein synthesis and stabilizes synapse-specific long-term facilitation in aplysia.

Protein type: Translation; RNA-binding

Chromosomal Location of Human Ortholog: 15q25.2

Cellular Component: postsynaptic membrane; dendrite; postsynaptic density; cytoplasm; nucleus; cell junction

Molecular Function: metal ion binding; translation repressor activity, nucleic acid binding; nucleotide binding

Biological Process: synaptonemal complex assembly; cellular response to insulin stimulus; mRNA processing; regulation of neuronal synaptic plasticity