Rabbit COX8A Polyclonal Antibody | anti-COX8A antibody

COX8A Polyclonal Antibody

Cat. Num. AAA3006539

• Gene Names: COX8A; COX; COX8; VIII; COX8L; COX8-2; VIII-L
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: COX8A; Polyclonal Antibody; COX8A Polyclonal Antibody; Cytochrome c oxidase subunit 8A; mitochondrial; Cytochrome c oxidase polypeptide VIII-liver/heart; Cytochrome c oxidase subunit 8-2; COX8; COX8L; anti-COX8A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: COX8A polyclonal antibody detects endogenous levels of COX8A protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 69

• Applicable Applications for anti-COX8A antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human COX8A.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of COX8A polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:sp2/0 whole cell lysateLane3:Rat brain tissue lysate)

Related Product Information for anti-COX8A antibody

The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water and ATP. The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some of which are mitochondrial and some of which are nuclear. The COX8 (cytochrome c oxidase subunit VIII) subunits are nuclear and have muscle and non-muscle-specific isoforms. COX8 exists as three isoforms: COX8a, a liver and heart isoform, Cox8b, a heart-specific isoform, and Cox8c, whose expression pattern has yet to be elucidated. All three Cox8 isoforms exists as components of the COX complex and play an important role in electron transport.

NCBI and Uniprot Product Information

• NCBI GI #: 4758044
• NCBI GeneID: 1351
• NCBI Accession #: NP_004065.1
• NCBI GenBank Nucleotide #: NP_004065.1
• UniProt Accession #: P10176
• Molecular Weight: ~ 8kDa
• NCBI Official Full Name: cytochrome c oxidase subunit 8A, mitochondrial
• NCBI Official Synonym Full Names: cytochrome c oxidase subunit 8A
• NCBI Official Symbol: COX8A
• NCBI Official Synonym Symbols: COX; COX8; VIII; COX8L; COX8-2; VIII-L
• NCBI Protein Information: cytochrome c oxidase subunit 8A, mitochondrial
• UniProt Protein Name: Cytochrome c oxidase subunit 8A, mitochondrial
• UniProt Gene Name: COX8A
• UniProt Synonym Gene Names: COX8; COX8L
• UniProt Entry Name: COX8A_HUMAN

NCBI Description

The protein encoded by this gene is the terminal enzyme of the respiratory chain, coupling the transfer of electrons from cytochrome c to molecular oxygen, with the concomitant production of a proton electrochemical gradient across the inner mitochondrial membrane. In addition to 3 mitochondrially encoded subunits, which perform the catalytic function, the eukaryotic enzyme contains nuclear-encoded smaller subunits, ranging in number from 4 in some organisms to 10 in mammals. It has been proposed that nuclear-encoded subunits may be involved in the modulation of the catalytic function. This gene encodes one of the nuclear-encoded subunits. [provided by RefSeq, Jul 2008]

Uniprot Description

COX8A: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Belongs to the cytochrome c oxidase VIII family.

Protein type: Energy Metabolism - oxidative phosphorylation; Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q12-q13

Cellular Component: mitochondrial inner membrane; integral to membrane

Molecular Function: cytochrome-c oxidase activity

Biological Process: cellular metabolic process; generation of precursor metabolites and energy; transmembrane transport