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Immunohistochemistry (IHC) (Immunochemical staining of human COX4NB in human skeletal muscle with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Rabbit anti-Human COX4NB Polyclonal Antibody | anti-EMC8 antibody

Anti-COX4NB Antibody, Rabbit Polyclonal

Gene Names
EMC8; NOC4; COX4NB; C16orf2; C16orf4; FAM158B
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
COX4NB; Polyclonal Antibody; Anti-COX4NB Antibody; Rabbit Polyclonal; EMC8/COX4NB/NOC4 Antibody; Rabbit PAb; Antigen Affinity Purified; ER membrane protein complex subunit 8; Anti-C16orf2 Antibody; Anti-C16orf4 Antibody; Anti-FAM158B Antibody; Anti-NOC4 Antibody; anti-EMC8 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human EMC8/COX4NB/NOC4
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-EMC8 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:1000-1:4000
IP: 1-4uL/mg of lysate
Immunogen
Recombinant Human EMC8/COX4NB/NOC4 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human EMC8/COX4NB/NOC4 (rh EMC8/COX4NB/NOC4). EMC8/COX4NB/NOC4 specific IgG was purified by Human EMC8/COX4NB/NOC4 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human COX4NB in human skeletal muscle with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC) (Immunochemical staining of human COX4NB in human skeletal muscle with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human COX4NB in human placenta with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC) (Immunochemical staining of human COX4NB in human placenta with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human COX4NB in human pancreas with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC) (Immunochemical staining of human COX4NB in human pancreas with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Western Blot (WB)

(Anti-COX4NB rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:24 kDaObserved band size:24 kDa(We are unsure as to the identity of these extra bands.))

Western Blot (WB) (Anti-COX4NB rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:24 kDaObserved band size:24 kDa(We are unsure as to the identity of these extra bands.))

Immunoprecipitation (IP)

(COX4NB was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-COX4NB rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-COX4NB rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size: 24 kDa)

Immunoprecipitation (IP) (COX4NB was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-COX4NB rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-COX4NB rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size: 24 kDa)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
210
NCBI Official Full Name
ER membrane protein complex subunit 8
NCBI Official Synonym Full Names
ER membrane protein complex subunit 8
NCBI Official Symbol
EMC8
NCBI Official Synonym Symbols
NOC4; COX4NB; C16orf2; C16orf4; FAM158B
NCBI Protein Information
ER membrane protein complex subunit 8; COX4 neighbor; neighbor of COX4; family with sequence similarity 158, member B
UniProt Protein Name
ER membrane protein complex subunit 8
UniProt Gene Name
EMC8
UniProt Synonym Gene Names
C16orf2; C16orf4; COX4AL; COX4NB; FAM158B; NOC4
UniProt Entry Name
EMC8_HUMAN

Uniprot Description

COX4NB: Belongs to the EMC8/EMC9 family.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 16q24

Cellular Component: mitochondrion; membrane; cytoplasm; nucleus

Research Articles on EMC8

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Product Notes

The EMC8 emc8 (Catalog #AAA8107464) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-COX4NB Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COX4NB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:1000-1:4000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the EMC8 emc8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COX4NB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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