Western Blot (WB)
(Anti-COX2/Cyclooxygenase 2 antibody, MBS175390, Western blottingLane 1: MCF-7 Cell LysateLane 2: HELA Cell LysateLane 3: MM453 Cell LysateLane 4: JURKAT Cell LysateLane 5: COLO320 Cell Lysate )
Rabbit COX2/Cyclooxygenase 2 Polyclonal Antibody | anti-PTGS2 antibody
Anti-COX2/Cyclooxygenase 2 antibody
Predicted to work with: Mouse
Predicted to work with: Mouse
Concentration: 0.1-0.5ug/ml
Tested Species:
Human, Rat
Predicted Species: Mouse
Immunohistochemistry (Paraffin-embedded Section) (IHC-P):
Concentration: 0.5-1ug/ml
Tested Species:
Human
Antigen Retrieval: By Heat
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate cuffer, pH6.0, for 20 mins is required for the staining of formalin/praffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Western Blot (WB)
(Anti-COX2/Cyclooxygenase 2 antibody, MBS175390, Western blottingLane 1: MCF-7 Cell LysateLane 2: HELA Cell LysateLane 3: MM453 Cell LysateLane 4: JURKAT Cell LysateLane 5: COLO320 Cell Lysate )
Western Blot (WB)
(Anti-COX2/Cyclooxygenase 2 antibody, MBS175390, Western blottingLane 1: MCF-7 Cell LysateLane 2: HELA Cell LysateLane 3: MM453 Cell LysateLane 4: JURKAT Cell LysateLane 5: COLO320 Cell Lysate )
Immunohistochemistry (IHC)
(Anti-COX2/Cyclooxygenase 2 antibody, MBS175390, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-COX2/Cyclooxygenase 2 antibody, MBS175390, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Background: Cyclooxygenase(Cox) is the key enzyme in conversion of arachidonic acid to PGs, and two isoforms, Cox-1 and Cox-2, have been identified. Cox-2 gene encodes an inducible prostaglandin synthase enzyme that is overexpressed in adenocarcinomas and other tumors. Deletion of the murine Cox-2 gene in Min mice reduced the incidence of intestinal tumors, suggesting that it is required for tumorigenesis. This gene is localized to sites associated with retinal blood vessels, and plays an important role in blood vessel formation in the retina. And the glucocorticoid receptor suppression of COX-2 is also crucial for curtailing lethal immune activation, and suggest new therapeutic approaches for regulation of T-cell-mediated inflammatory diseases.
NCBI and Uniprot Product Information
NCBI Description
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]
Uniprot Description
COX-2: Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Homodimer. Belongs to the prostaglandin G/H synthase family.
Protein type: Oxidoreductase; Lipid Metabolism - arachidonic acid; EC 1.14.99.1
Chromosomal Location of Human Ortholog: 1q25.2-q25.3
Cellular Component: endoplasmic reticulum membrane; neuron projection; protein complex; cytoplasm; caveola; nucleus
Molecular Function: prostaglandin-endoperoxide synthase activity; enzyme binding; peroxidase activity; protein homodimerization activity; arachidonate 15-lipoxygenase activity; metal ion binding; heme binding; lipid binding
Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; response to lithium ion; positive regulation of smooth muscle cell proliferation; response to glucocorticoid stimulus; response to lipopolysaccharide; positive regulation of NF-kappaB import into nucleus; decidualization; sensory perception of pain; prostaglandin biosynthetic process; negative regulation of synaptic transmission, dopaminergic; positive regulation of synaptic transmission, glutamatergic; response to estradiol stimulus; bone mineralization; negative regulation of cell cycle; negative regulation of cell proliferation; response to vitamin D; lipoxygenase pathway; nicotinamide metabolic process; negative regulation of smooth muscle contraction; regulation of blood pressure; arachidonic acid metabolic process; positive regulation of smooth muscle contraction; angiogenesis; inflammatory response; response to fructose stimulus; water-soluble vitamin metabolic process; positive regulation of prostaglandin biosynthetic process; response to drug; negative regulation of calcium ion transport; vitamin metabolic process; cyclooxygenase pathway; positive regulation of synaptic plasticity; learning; memory; prostaglandin metabolic process; hair cycle; response to manganese ion; ovulation; NAD metabolic process; regulation of inflammatory response; positive regulation of vasoconstriction; positive regulation of fever; brown fat cell differentiation; response to oxidative stress; cell motility; embryo implantation
Research Articles on PTGS2
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Product Notes
The PTGS2 ptgs2 (Catalog #AAA175390) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-COX2/Cyclooxygenase 2 antibody reacts with Reacts with: Human, Rat Predicted to work with: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's COX2/Cyclooxygenase 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot (WB): Concentration: 0.1-0.5ug/ml Tested Species: Human, Rat Predicted Species: Mouse Immunohistochemistry (Paraffin-embedded Section) (IHC-P): Concentration: 0.5-1ug/ml Tested Species: Human Antigen Retrieval: By Heat Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate cuffer, pH6.0, for 20 mins is required for the staining of formalin/praffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the PTGS2 ptgs2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COX2/Cyclooxygenase 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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