Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse RILP-like protein 2 (RILPL2) Standard Curve.)
Mouse RILP-Like Protein 2 (RILPL2) ELISA Kit | RILPL2 elisa kit
Mouse RILP-Like Protein 2 (RILPL2) ELISA Kit
Reactivity
Mouse
Synonyms
RILP-Like Protein 2 (RILPL2); Mouse RILP-Like Protein 2 (RILPL2) ELISA Kit; RILPL2; FLJ30380; FLJ32372; MGC7036; RILPL2 elisa kit
Reactivity
Mouse
Specificity
Mouse RILP-like protein 2 (RILPL2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse RILPL2. No significant cross-reactivity or interference between Mouse RILPL2 and analogues was observed.
Sequence Length
197
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse RILP-like protein 2 (RILPL2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse RILP-like protein 2 (RILPL2) Standard Curve.)
Related Product Information for RILPL2 elisa kit
Description: This Mouse RILP-like protein 2 (RILPL2) ELISA Kit employs a two-site sandwich ELISA to quantitate RILPL2 in samples. An antibody specific for RILPL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RILPL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RILPL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RILPL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: RILPL2 regulates cellular morphology. Knockdown of endogenous RILPL2 in neurons by short hairpin RNA (shRNA) interference results in reduced spine-like protrusions, a phenotype rescued by overexpression of an shRNA-insensitive RILPL2 mutant, suggesting a role for RILPL2 in both the establishment and maintenance of dendritic spines. RILPL2 and the Rho GTPase Rac1 form a complex, and that RILPL2 is able to induce activation of Rac1 and its target, p21-activated kinase (Pak). Notably, both RILPL2-mediated morphological changes and activation of Rac1-Pak signaling were blocked by expression of a truncated tail form of MyoVa or MyoVa shRNA, demonstrating that MyoVa is crucial for proper RILPL2 function. This might represent a novel mechanism linking RILPL2, the motor protein MyoVa and Rac1 with neuronal structure and function
Background: RILPL2 regulates cellular morphology. Knockdown of endogenous RILPL2 in neurons by short hairpin RNA (shRNA) interference results in reduced spine-like protrusions, a phenotype rescued by overexpression of an shRNA-insensitive RILPL2 mutant, suggesting a role for RILPL2 in both the establishment and maintenance of dendritic spines. RILPL2 and the Rho GTPase Rac1 form a complex, and that RILPL2 is able to induce activation of Rac1 and its target, p21-activated kinase (Pak). Notably, both RILPL2-mediated morphological changes and activation of Rac1-Pak signaling were blocked by expression of a truncated tail form of MyoVa or MyoVa shRNA, demonstrating that MyoVa is crucial for proper RILPL2 function. This might represent a novel mechanism linking RILPL2, the motor protein MyoVa and Rac1 with neuronal structure and function
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
RILP-like protein 2
NCBI Official Synonym Full Names
Rab interacting lysosomal protein-like 2
NCBI Official Symbol
Rilpl2
NCBI Protein Information
RILP-like protein 2
UniProt Protein Name
RILP-like protein 2
Protein Family
UniProt Gene Name
Rilpl2
UniProt Entry Name
RIPL2_MOUSE
Uniprot Description
RILPL2: Involved in cell shape and neuronal morphogenesis, positively regulating the establishment and maintenance of dendritic spines. May activate RAC1. Belongs to the RILPL family.
Protein type: Unknown function
Cellular Component: centrosome; cell projection; cytoskeleton; cell; cytoplasm; intracellular; cilium
Molecular Function: identical protein binding
Biological Process: intracellular protein transport; protein transport; transport
Research Articles on RILPL2
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Product Notes
The Mouse RILPL2 rilpl2 (Catalog #AAA9717109) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717109 ELISA Kit recognizes Mouse RILPL2. It is sometimes possible for the material contained within the vial of "RILP-Like Protein 2 (RILPL2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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