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Western Blot (WB)
(Western blot analysis of extracts of normal 293T cells 293T transfected with Spike S2 ECD Protein, using SARS-CoV-2 Spike S2 ECD antibody at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 1s.)
Rabbit anti-SARS-CoV-2 COVID 19 Spike S2 ECD Coronavirus Polyclonal Antibody | anti-SARS-CoV-2 antibody
SARS-CoV-2 Spike S2 ECD Rabbit pAb
Reactivity
SARS-CoV-2
Applications
ELISA, Western Blot, Immunofluorescence, Immunoprecipitation
Purity
Affinity purification
Synonyms
COVID 19 Spike S2 ECD Coronavirus; Polyclonal Antibody; SARS-CoV-2 Spike S2 ECD Rabbit pAb; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Nucleocapsid; Nucleoprotein (NP); Spike S2 ECD; anti-SARS-CoV-2 antibody
Host
Rabbit
Reactivity
SARS-CoV-2
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applicable Applications for anti-SARS-CoV-2 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
ELISA: 1:500-1:2000
WB: 1:500-1:2000
FC: 1:50-1:200
IF: 1:50-1:200
WB: 1:500-1:2000
FC: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant fusion protein of SARS-CoV-2 Spike S2 ECD.
Positive Samples
293T
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of normal 293T cells 293T transfected with Spike S2 ECD Protein, using SARS-CoV-2 Spike S2 ECD antibody at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 1s.)
Western Blot (WB)
(Western blot analysis of extracts of normal 293T cells 293T transfected with Spike S2 ECD Protein, using SARS-CoV-2 Spike S2 ECD antibody at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 1s.)
Testing Data
(Immobilized Recombinant SARS-COV2 S-ECD-PFS Protein(RP01283LQ) at 1 ug/mL (100 uL/well) can bind SARS-CoV-2 Spike S2 ECD Rabbit pAb with a linear range of 3.12-50ng/mL.)
Testing Data
(Immobilized Recombinant SARS-COV2 S-ECD-PFS Protein(RP01283LQ) at 1 ug/mL (100 uL/well) can bind SARS-CoV-2 Spike S2 ECD Rabbit pAb with a linear range of 3.12-50ng/mL.)
Immunofluorescence (IF)
(Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 Spike S2 ECD fusion protein (top left) and untreated 293T cells (top right) use SARS-CoV-2 Spike S2 ECD Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 Spike S2 ECD fusion protein (top left) and untreated 293T cells (top right) use SARS-CoV-2 Spike S2 ECD Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunopreciptation (IP)
(Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug SARS-CoV-2 Spike S2 ECD antibody . Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike S2 ECD antibody at a dilition of 1:5000.)
Immunopreciptation (IP)
(Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug SARS-CoV-2 Spike S2 ECD antibody . Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike S2 ECD antibody at a dilition of 1:5000.)
NCBI and Uniprot Product Information
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Product Notes
The SARS-CoV-2 (Catalog #AAA9143563) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 Spike S2 ECD Rabbit pAb reacts with SARS-CoV-2 and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 Spike S2 ECD Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). ELISA: 1:500-1:2000 WB: 1:500-1:2000 FC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the SARS-CoV-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Spike S2 ECD Coronavirus, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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