Western Blot (WB)
(Western blot analysis of extracts from Hepg2, using NDR1/2 (Phospho-Thr444/442) Antibody. Lane 1 was treated with the blocking peptide.)
Rabbit NDR1/2 Polyclonal Antibody | anti-NDR1/2 antibody
Phospho-NDR1/2 (Thr444/Thr442) Antibody
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Tissue Specificity: Ubiquitously expressed with highest levels observed in the thymus.
IHC: 1:50-1:200
Peptide ELISA: 1:20,000-1:40,000
Note: Associated with the actin cytoskeleton. Co-localizes with STK24/MST3 in the membrane.
Western Blot (WB)
(Western blot analysis of extracts from Hepg2, using NDR1/2 (Phospho-Thr444/442) Antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Hepg2, using NDR1/2 (Phospho-Thr444/442) Antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis NDR1/2 (Phospho-Thr444/442) using EGF treated HepG2 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Western Blot (WB)
(Western blot analysis NDR1/2 (Phospho-Thr444/442) using EGF treated HepG2 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Immunohistochemistry (IHC)
(At 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the AGC serine/threonine kinase family of proteins. The kinase activity of this protein is regulated by autophosphorylation and phosphorylation by other upstream kinases. This protein has been shown to function in the cell cycle and apoptosis. This protein has also been found to regulate the protein stability and transcriptional activity of the MYC oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]
Uniprot Description
NDR1: is an AGC kinase of the NDR family. An apparent upstream regulator of Myc activity in human B-cell lymphoma. Mediates apoptosis downstream of the tumor suppressor proteins RASSF1A and MST1. Reduced expression of NDR1 results in defective apoptotic responses and a predisposition to develop T cell lymphoma in mice. Activated by binding of S100B which releases autoinhibitory N-lobe interactions. Ubiquitously expressed with highest levels observed in peripheral blood leukocytes.
Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, AGC; AGC group; NDR family
Chromosomal Location of Human Ortholog: 6p21
Cellular Component: cytoplasm; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; magnesium ion binding; mitogen-activated protein kinase kinase kinase binding; ATP binding
Biological Process: negative regulation of MAP kinase activity; protein modification process; protein amino acid phosphorylation
Research Articles on NDR1/2
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Product Notes
The NDR1/2 stk38 (Catalog #AAA9614175) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-NDR1/2 (Thr444/Thr442) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's NDR1/2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). WB: 1:1000-3000 IHC: 1:50-1:200 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the NDR1/2 stk38 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDR1/2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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