Immunohistochemistry (IHC)
(Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP16 in COVID-19 Patient LungImmunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP16 antibody (9271, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP16 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.)
Rabbit COVID 19 NSP16 Coronavirus Polyclonal Antibody | anti-COVID-19 antibody
SARS-CoV-2 (COVID-19) NSP16 Antibody
Reactivity
VirusPredicted reactivity based on immunogen sequence: SARS-CoV NSP16 protein: (88%)
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
SARS-CoV-2 (COVID-19) NSP16 Antibody is affinity chromatography purified via peptide column.
Synonyms
COVID 19 NSP16 Coronavirus; Polyclonal Antibody; SARS-CoV-2 (COVID-19) NSP16 Antibody; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; NSP16; 2'-O-methyltransferase; nsp16; Non-structure protein 16; ORF1a polyprotein; ORF1ab polyprotein; anti-COVID-19 antibody
Host
Rabbit
Reactivity
Virus
Predicted reactivity based on immunogen sequence: SARS-CoV NSP16 protein: (88%)
Predicted reactivity based on immunogen sequence: SARS-CoV NSP16 protein: (88%)
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
SARS-CoV-2 (COVID-19) NSP16 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid. SARS-CoV-2 (COVID-19) NSP16 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
IHC: 0.5ug/mL
WB: 0.5ug/mL
Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) NSP16 antibody can detect 2ng of free peptide at 1ug/mL in ELISA. It can detect SARS-CoV-2 NSP16 recombinant protein by ELISA and WB.
WB: 0.5ug/mL
Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) NSP16 antibody can detect 2ng of free peptide at 1ug/mL in ELISA. It can detect SARS-CoV-2 NSP16 recombinant protein by ELISA and WB.
Organism
Severe acute respiratory syndrome coronavirus 2
Immunogen
Anti-SARS-CoV-2 (COVID-19) NSP16 antibody was raised against a peptide corresponding to 16 amino acids near the central domain of SARS-CoV-2 (COVID-19) NSP16 protein.
The immunogen is located within 120-170 amino acids of the SARS-CoV-2 (COVID-19) NSP16 protein.
The immunogen is located within 120-170 amino acids of the SARS-CoV-2 (COVID-19) NSP16 protein.
Conjugation
Unconjugated
Protein GI#
1819735431
Preparation and Storage
SARS-CoV-2 (COVID-19) NSP16 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Immunohistochemistry (IHC)
(Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP16 in COVID-19 Patient LungImmunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP16 antibody (9271, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP16 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.)
Immunohistochemistry (IHC)
(Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP16 in COVID-19 Patient LungImmunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP16 antibody (9271, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP16 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.)
ELISA (EIA)
(Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) NSP16 Protein Antibodies: SARS-CoV-2 (COVID-19) NSP16 Antibody, 9271. A direct ELISA was performed using SARS-CoV-2 NSP16 recombinant protein (10-418) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP16 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/ml)
ELISA (EIA)
(Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) NSP16 Protein Antibodies: SARS-CoV-2 (COVID-19) NSP16 Antibody, 9271. A direct ELISA was performed using SARS-CoV-2 NSP16 recombinant protein (10-418) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP16 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/ml)
Western Blot (WB)
(Figure 3 Western Blot Validation with SARS-CoV-2 (COVID-19) NSP16 Protein</strong><br>Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP16 recombinant protein. Antibodies: SARS-CoV-2 (COVID-19) NSP16, 9271, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 μg/mL and Lane 2: 2 μg/mL)
Western Blot (WB)
(Figure 3 Western Blot Validation with SARS-CoV-2 (COVID-19) NSP16 Protein</strong><br>Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP16 recombinant protein. Antibodies: SARS-CoV-2 (COVID-19) NSP16, 9271, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 μg/mL and Lane 2: 2 μg/mL)
Product Categories/Family for anti-COVID-19 antibody
References
Gorbalenya. bioRxiv: 2020.
Hui et al. Int J Infect Dis. 2020;91:264-266.
Hui et al. Int J Infect Dis. 2020;91:264-266.
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Product Notes
The COVID-19 (Catalog #AAA154760) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 (COVID-19) NSP16 Antibody reacts with Virus Predicted reactivity based on immunogen sequence: SARS-CoV NSP16 protein: (88%) and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 NSP16 Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). IHC: 0.5ug/mL WB: 0.5ug/mL Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) NSP16 antibody can detect 2ng of free peptide at 1ug/mL in ELISA. It can detect SARS-CoV-2 NSP16 recombinant protein by ELISA and WB. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 NSP16 Coronavirus, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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