Western Blot (WB)
(Western Blot: A431 cell lysate was probed with MBS614430 (0.1ug/ml). )
Rabbit CoREST Polyclonal Antibody | anti-CoREST antibody
CoREST (REST corepressor 1, Protein CoREST, KIAA0071, RCOR, RCOR1) (APC)
Gene Names
RCOR1; RCOR; COREST; KIAA0071
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CoREST; Polyclonal Antibody; CoREST (REST corepressor 1; Protein CoREST; KIAA0071; RCOR; RCOR1) (APC); anti-CoREST antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CoREST, Mr ~66kD. Species Crossreactivity: Mouse and rat.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-CoREST antibody
Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
ICC: Positive immunostaining for CoREST in cells fixed with 4% paraformaldehyde pH 7.3 for 10-15 minutes
WB: 0.1-0.5ug/ml detects CoREST in RIPA lysates from A431, 3T3/A31, Jurkat, and Hela cells. Reported to be detected in RIPA lysates from HEK 293, L6, PC12, C6 cells and liver, muscle, heart and brain tissue.
IHC: Reported to detect CoREST in tissue sections.
IP: 2.5ug immunoprecipitates CoREST from 250ug of A431 RIPA lysate.
Applications are based on unconjugated antibody.
WB: 0.1-0.5ug/ml detects CoREST in RIPA lysates from A431, 3T3/A31, Jurkat, and Hela cells. Reported to be detected in RIPA lysates from HEK 293, L6, PC12, C6 cells and liver, muscle, heart and brain tissue.
IHC: Reported to detect CoREST in tissue sections.
IP: 2.5ug immunoprecipitates CoREST from 250ug of A431 RIPA lysate.
Applications are based on unconjugated antibody.
Immunogen
GST fusion protein corresponding to residues 109-293 of human CoREST.
Conjugate
APC
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot: A431 cell lysate was probed with MBS614430 (0.1ug/ml). )
Western Blot (WB)
(Western Blot: A431 cell lysate was probed with MBS614430 (0.1ug/ml). )
Product Categories/Family for anti-CoREST antibody
References
1. Lunyak, V. V., et. al., Science 298: 1747-1752, 2003. 2. Ballas, N., et. al., Neuron 31: 353-365, 2001. 3. Andres, M. E., et. al., PNAS 96: 9873-9878, 1999.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,028 Da
NCBI Official Full Name
REST corepressor 1
NCBI Official Synonym Full Names
REST corepressor 1
NCBI Official Symbol
RCOR1
NCBI Official Synonym Symbols
RCOR; COREST; KIAA0071
NCBI Protein Information
REST corepressor 1
UniProt Protein Name
REST corepressor 1
Protein Family
UniProt Gene Name
RCOR1
UniProt Synonym Gene Names
KIAA0071; RCOR
UniProt Entry Name
RCOR1_HUMAN
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Product Notes
The CoREST rcor1 (Catalog #AAA6470289) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CoREST (REST corepressor 1, Protein CoREST, KIAA0071, RCOR, RCOR1) (APC) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CoREST can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). ICC: Positive immunostaining for CoREST in cells fixed with 4% paraformaldehyde pH 7.3 for 10-15 minutes WB: 0.1-0.5ug/ml detects CoREST in RIPA lysates from A431, 3T3/A31, Jurkat, and Hela cells. Reported to be detected in RIPA lysates from HEK 293, L6, PC12, C6 cells and liver, muscle, heart and brain tissue. IHC: Reported to detect CoREST in tissue sections. IP: 2.5ug immunoprecipitates CoREST from 250ug of A431 RIPA lysate. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CoREST rcor1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CoREST, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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