Rabbit anti-Human CRY2 Polyclonal Antibody | anti-CRY2 antibody

CRY2 polyclonal antibody

Cat. Num. AAA3015861

• Gene Names: CRY2; HCRY2; PHLL2
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Synonyms: CRY2; Polyclonal Antibody; CRY2 polyclonal antibody; Cryptochrome-2; anti-CRY2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: CRY2 polyclonal antibody detects endogenous levels of CRY2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Applicable Applications for anti-CRY2 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000~1:2000
• Immunogen: Synthetic peptide, corresponding to Human CRY2.
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of CRY2 polyclonal antibody at 1:2000 dilution Lane1:A549 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:U-87MG whole cell lysate(40ug) Lane4:K562 whole cell lysate(40ug))

Related Product Information for anti-CRY2 antibody

Circadian clocks are biological timepieces that regulate hormonal rhythms, sleep cycles and feeding behaviors. These rhythms are generated in the superchiasmatic nucleus (SCN), a cell-autonomous circadian oscillator located within the brain that is synchronized with the environment by light. A number of transcription factors, including Clock and BMAL1, are molecular components of the SCN that induce the expression of proteins involved in light/dark cycle entrainment, which include Per1 and Per2. Tim, for timeless, generates a negative feedback loop that regulates the activity of Clock by suppressing the expression of Clock target genes. Tim forms heterodimers with Per1 and Per2 that bind Clock and block the activation of Clock-BMAL1 dimers to repress Per gene expression. Additionally, the CRY proteins, which are cryptochrome photoreceptors for the circadian clock, function as light-independent inhibitors of the circadian clock. CRY1 and CRY2 negatively regulate SCN components by associating with the activators Clock-BMAL1, and also with the various feedback inhibitors Per1, Per2 and Tim.

NCBI and Uniprot Product Information

• NCBI GI #: 118572252
• NCBI GeneID: 1408
• UniProt Accession #: Q49AN0; O75148; Q8IV71; B4DH32; B4DZD6
• NCBI Official Full Name: Cryptochrome-2
• NCBI Official Synonym Full Names: cryptochrome circadian clock 2
• NCBI Official Symbol: CRY2
• NCBI Official Synonym Symbols: HCRY2; PHLL2
• NCBI Protein Information: cryptochrome-2; growth-inhibiting protein 37; cryptochrome 2 (photolyase-like)
• UniProt Protein Name: Cryptochrome-2
• Protein Family: Cryptochrome
• UniProt Gene Name: CRY2
• UniProt Synonym Gene Names: KIAA0658
• UniProt Entry Name: CRY2_HUMAN

NCBI Description

This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]

Uniprot Description

CRY2: Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL. Belongs to the DNA photolyase class-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Lyase

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: cytoplasm; extracellular region; nucleus

Molecular Function: ubiquitin binding; protein binding; DNA binding; DNA (6-4) photolyase activity; damaged DNA binding; deoxyribodipyrimidine photo-lyase activity; blue light photoreceptor activity; single-stranded DNA binding; phosphatase binding

Biological Process: circadian rhythm; photoreactive repair; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; regulation of circadian rhythm; protein-chromophore linkage; entrainment of circadian clock by photoperiod; blue light signaling pathway; negative regulation of phosphoprotein phosphatase activity; negative regulation of circadian rhythm; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent