Rabbit anti-Human COPS8 Polyclonal Antibody | anti-COPS8 antibody

COPS8, NT (COPS8, CSN8, COP9 signalosome complex subunit 8, COP9 homolog, JAB1-containing signalosome subunit 8) (Azide free) (HRP)

Cat. Num. AAA6287904

• Gene Names: COPS8; COP9; CSN8; SGN8
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: COPS8; Polyclonal Antibody; NT (COPS8; CSN8; COP9 signalosome complex subunit 8; COP9 homolog; JAB1-containing signalosome subunit 8) (Azide free) (HRP); JAB1-containing signalosome subunit 8; anti-COPS8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-COPS8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: COPS8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human COPS8.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(COPS8 Antibody (N-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the COPS8 antibody detected the COPS8 protein (arrow).)

Related Product Information for anti-COPS8 antibody

The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Product Categories/Family for anti-COPS8 antibody

Antibodies; Signal Transduction

References

Enchev, R.I., et al. Structure 18(4):518-527(2010). Miyauchi, Y., et al. J. Biol. Chem. 283(24):16622-16631(2008). Schweitzer, K., et al. EMBO J. 26(6):1532-1541(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Wang, Y., et al. FEBS Lett. 572 (1-3), 85-91 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 5729779
• NCBI GeneID: 10920
• NCBI Accession #: NP_006701.1; NP_937832.1
• NCBI GenBank Nucleotide #: NM_006710.4
• UniProt Accession #: Q99627; Q53QS9; A8K1H6
• Molecular Weight: 17,909 Da
• NCBI Official Full Name: COP9 signalosome complex subunit 8 isoform 1
• NCBI Official Synonym Full Names: COP9 signalosome subunit 8
• NCBI Official Symbol: COPS8
• NCBI Official Synonym Symbols: COP9; CSN8; SGN8
• NCBI Protein Information: COP9 signalosome complex subunit 8; COP9 constitutive photomorphogenic homolog subunit 8; COP9 homolog; JAB1-containing signalosome subunit 8; hCOP9
• UniProt Protein Name: COP9 signalosome complex subunit 8
• UniProt Gene Name: COPS8
• UniProt Synonym Gene Names: CSN8; SGN8; Signalosome subunit 8; hCOP9
• UniProt Entry Name: CSN8_HUMAN

NCBI Description

The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

COPS8: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Belongs to the CSN8 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: signalosome; nucleoplasm; perinuclear region of cytoplasm; cytoplasm; nucleus

Biological Process: negative regulation of cell proliferation; cullin deneddylation; activation of NF-kappaB-inducing kinase