Rabbit anti-Human COPE Polyclonal Antibody | anti-COPE antibody

COPE, CT (COPE, Coatomer subunit epsilon, Epsilon-coat protein) (PE)

Cat. Num. AAA6287866

• Gene Names: COPE; epsilon-COP
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: COPE; Polyclonal Antibody; CT (COPE; Coatomer subunit epsilon; Epsilon-coat protein) (PE); Epsilon-coat protein; anti-COPE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-COPE antibody: Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: COPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280-308 amino acids from the C-terminal region of human COPE.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(COPE Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the COPE antibody detected the COPE protein (arrow).)

Immunohistochemistry (IHC)

(COPE Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of COPE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of COPE Antibody (C-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-COPE antibody

The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product Categories/Family for anti-COPE antibody

Antibodies; Proteins

References

Davila, S., et al. Genes Immun. 11(3):232-238(2010). Morikawa, R.K., et al. J. Biol. Chem. 284(39):26620-26630(2009). Maruyama, S., et al. Mol. Cell. Biochem. 307 (1-2), 73-82 (2008): Goryachev, A.B., et al. PLoS Comput. Biol. 2 (12), E172 (2006): Lippincott-Schwartz, J., et al. Trends Cell Biol. 16 (10), E1-E4 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 31542319
• NCBI GeneID: 11316
• NCBI Accession #: NP_009194.2
• NCBI GenBank Nucleotide #: NM_007263.3
• UniProt Accession #: O14579; O76097; Q6IBB8; Q9UGP6; A6NE29; A6NKA3
• Molecular Weight: 28,773 Da
• NCBI Official Full Name: coatomer subunit epsilon isoform a
• NCBI Official Synonym Full Names: coatomer protein complex subunit epsilon
• NCBI Official Symbol: COPE
• NCBI Official Synonym Symbols: epsilon-COP
• NCBI Protein Information: coatomer subunit epsilon
• UniProt Protein Name: Coatomer subunit epsilon
• Protein Family: Coatomer
• UniProt Gene Name: COPE
• UniProt Synonym Gene Names: Epsilon-COP
• UniProt Entry Name: COPE_HUMAN

Uniprot Description

COPE: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Belongs to the COPE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: COPI vesicle coat; cytosol; endoplasmic reticulum membrane; Golgi apparatus; Golgi membrane; nucleoplasm; transport vesicle

Molecular Function: protein binding

Biological Process: ER to Golgi vesicle-mediated transport; intra-Golgi vesicle-mediated transport; retrograde vesicle-mediated transport, Golgi to ER