Rabbit anti-Human Connexin 37 Polyclonal Antibody | anti-Cx37 antibody

Connexin 37, CT (Gap Junction alpha-4 Protein, GJA4, Connexin-37, Cx37) (APC)

Cat. Num. AAA6470124

• Gene Names: GJA4; CX37
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Connexin 37; Polyclonal Antibody; CT (Gap Junction alpha-4 Protein; GJA4; Connexin-37; Cx37) (APC); anti-Cx37 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GJA4.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Cx37 antibody: Immunohistochemistry (IHC), FLISA
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human GJA4 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GJA4 Antibody (K317).Connexin western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the GJA4 antibody detected the GJA4 protein (arrow).)

Immunohistochemistry (IHC)

(Connexin 37 Antibody (GJA4) (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Connexin 37 Antibody (GJA4) (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-Cx37 antibody

Antibodies; Connexin; Junction Proteins

References

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Saito, T., et al., Int. J. Cancer 86(1):67-70 (2000). 3. Boerma, M., et al., J. Intern. Med. 246(2):211-218 (1999). 4. Krutovskikh, V., et al., Carcinogenesis 17(8):1761-1763 (1996). 5. Reed, K.E., et al., J. Clin. Invest. 91(3):997-1004 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 62865885
• NCBI GeneID: 2701
• NCBI Accession #: NP_002051.2
• NCBI GenBank Nucleotide #: NM_002060.2
• UniProt Accession #: P35212; Q9P106; Q9UBL1; Q9UNA9; Q9UNB0; Q9UNB1; Q9Y5N7; A8K698; D3DPR4
• Molecular Weight: 37,414 Da
• NCBI Official Full Name: gap junction alpha-4 protein
• NCBI Official Synonym Full Names: gap junction protein, alpha 4, 37kDa
• NCBI Official Symbol: GJA4
• NCBI Official Synonym Symbols: CX37
• NCBI Protein Information: gap junction alpha-4 protein; connexin 37; connexin-37
• UniProt Protein Name: Gap junction alpha-4 protein
• UniProt Gene Name: GJA4
• UniProt Synonym Gene Names: Cx37
• UniProt Entry Name: CXA4_HUMAN

NCBI Description

This gene encodes a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene have been associated with atherosclerosis and a higher risk of myocardial infarction. [provided by RefSeq, Jul 2008]

Uniprot Description

GJA4: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Belongs to the connexin family. Alpha-type (group II) subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell adhesion

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: connexon complex; integral to plasma membrane; gap junction

Molecular Function: protein binding

Biological Process: blood vessel development; intercellular junction assembly; cell-cell signaling; transport; calcium ion transport; response to pain