anti-Mouse Connexin 31 Polyclonal Antibody | anti-GJB3 antibody
Anti - Connexin 31 (CX31, Gap Junction Beta 3 Protein, CXB-3)
Gene Names
GJB3; EKV; CX31; DFNA2; DFNA2B; FLJ22486; MGC102938
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
Connexin 31; Polyclonal Antibody; Anti - Connexin 31 (CX31; Gap Junction Beta 3 Protein; CXB-3); Connexin 31 (CX31; anti-GJB3 antibody
Host
Host: Rabbit
Source: Mouse
Source: Mouse
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
The mouse Cx31 immunogenic peptide sequence shows 3 aa differences in rat Cx 31 (4). The Cx31 peptide sequence does not significant homology with other known Connexins. Actual crossreactivity of antibodies in various species is not established
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Supplied as a liquid, neat serum, 40% glycerol.
Sequence Length
270
Applicable Applications for anti-GJB3 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blot. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. An antibody raised against a GST-fusion has been shown to recognize ~31kD band in skin (3).
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. An antibody raised against a GST-fusion has been shown to recognize ~31kD band in skin (3).
Immunogen
A 17 amino acid peptide sequence within the 1st cytoplasmic domain of the rat Cx31 (2) was used to generate anti-Mouse Cx31. Amino terminal Cysteine was added for conjugation to KLH (carrier protein)
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Product Categories/Family for anti-GJB3 antibody
References
• 1. Kumar, Nm and Giula, NB (1996) Cell 84, 381-388; Evans, HW (1994) Biochem. Soc. Tr. 788-792; Byer, E et al (1990) J. membrane Biol. 116, 187-1942. Henneman, H et al (1992) Eur. J. Cell Biol. 57, 51-583. Butterweck, A et al (1994) Eur. J. cell Biol. 65, 152-1634. Hoh, JH et al (1991) J. Biol. Chem. 266, 6524-6531
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,818 Da
NCBI Official Full Name
connexin 31
NCBI Official Synonym Full Names
gap junction protein, beta 3, 31kDa
NCBI Official Symbol
GJB3
NCBI Official Synonym Symbols
EKV; CX31; DFNA2; DFNA2B; FLJ22486; MGC102938
NCBI Protein Information
gap junction beta-3 protein; connexin 31; connexin-31; OTTHUMP00000004183; OTTHUMP00000004184
UniProt Protein Name
Gap junction beta-3 protein
Protein Family
UniProt Gene Name
GJB3
UniProt Synonym Gene Names
CX31
UniProt Entry Name
CXB3_HUMAN
Similar Products
Product Notes
The GJB3 gjb3 (Catalog #AAA615203) is an Antibody produced from Host: Rabbit Source: Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti - Connexin 31 (CX31, Gap Junction Beta 3 Protein, CXB-3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Connexin 31 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blot. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. An antibody raised against a GST-fusion has been shown to recognize ~31kD band in skin (3). Researchers should empirically determine the suitability of the GJB3 gjb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Connexin 31, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
