Human Phenylalanine Hydroxylase (PAH) RTU ELISA Kit | PAH rtu elisa kit

Human Phenylalanine Hydroxylase (PAH) ELISA Kit

Cat. Num. AAA4501634

• Gene Names: PAH; PH; PKU; PKU1
• Reactivity: Human
• Synonyms: Phenylalanine Hydroxylase (PAH); Human Phenylalanine Hydroxylase (PAH) ELISA Kit; PKU; PKU1; Phe-4-monooxygenase; PAH rtu elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PAH. No significant cross-reactivity or interference between PAH and analogues was observed.
• Sequence Length: 452

• Samples: Human tissue homogenates, cell lysates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.56ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PAH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PAH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PAH rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PAH in human tissue homogenates, cell lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PAH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PAH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PAH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PAH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557819
• NCBI GeneID: 5053
• NCBI Accession #: NP_000268.1
• NCBI GenBank Nucleotide #: NM_000277.2
• UniProt Accession #: P00439; Q16717; Q8TC14
• Molecular Weight: 51,862 Da
• NCBI Official Full Name: phenylalanine-4-hydroxylase
• NCBI Official Synonym Full Names: phenylalanine hydroxylase
• NCBI Official Symbol: PAH
• NCBI Official Synonym Symbols: PH; PKU; PKU1
• NCBI Protein Information: phenylalanine-4-hydroxylase
• UniProt Protein Name: Phenylalanine-4-hydroxylase
• Protein Family: Phenylalanine-4-hydroxylase
• UniProt Gene Name: PAH
• UniProt Synonym Gene Names: PAH

NCBI Description

This gene encodes a member of the biopterin-dependent aromatic amino acid hydroxylase protein family. The encoded phenylalanine hydroxylase enzyme hydroxylates phenylalanine to tyrosine and is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. [provided by RefSeq, Aug 2017]