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Western Blot (WB) (Western Blot: Sample: Recombinant CSF2Rb, Human.)

Rabbit anti-Human Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) Polyclonal Antibody | anti-CSF2Rb antibody

Polyclonal Antibody to Colony Stimulating Factor 2 Receptor Beta (CSF2Rb)

Gene Names
CSF2RB; CD131; IL3RB; IL5RB; SMDP5; CDw131; betaGMR
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Colony Stimulating Factor 2 Receptor Beta (CSF2Rb); Polyclonal Antibody; Polyclonal Antibody to Colony Stimulating Factor 2 Receptor Beta (CSF2Rb); anti-CSF2Rb antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CSF2Rb. It has been selected for its ability to recognize CSF2Rb in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- PPEPRDLQ ISTDQDHFLL TWSVALGSPQ SHWLSPGDLE FEVVYKRLQD SWEDAAILLS NTSQATLGPE HLMPSSTYVA RVRTRLAPGS RLSGRPSKWS PEV
Sequence Length
903
Applicable Applications for anti-CSF2Rb antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant CSF2Rb (Pro133~Val233) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2056272
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant CSF2Rb, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant CSF2Rb, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Endometrial cancer Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Endometrial cancer Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
97,976 Da
NCBI Official Full Name
cytokine receptor common subunit beta
NCBI Official Synonym Full Names
colony stimulating factor 2 receptor beta common subunit
NCBI Official Symbol
CSF2RB
NCBI Official Synonym Symbols
CD131; IL3RB; IL5RB; SMDP5; CDw131; betaGMR
NCBI Protein Information
cytokine receptor common subunit beta
UniProt Protein Name
Cytokine receptor common subunit beta
Protein Family
UniProt Gene Name
CSF2RB
UniProt Synonym Gene Names
IL3RB; IL5RB

NCBI Description

The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP). [provided by RefSeq, Jul 2008]

Uniprot Description

CSF2RB: a common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. A type I cytokine family of receptor. Defects are a cause of congenital pulmonary alveolar proteinosis (PAP) [MIM:265120]. PAP is an autosomal recessive fatal respiratory disease.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: granulocyte macrophage colony-stimulating factor receptor complex; integral component of plasma membrane; intracellular; plasma membrane

Molecular Function: interleukin-3 receptor activity; interleukin-5 receptor activity; protein binding; protein tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor activity

Biological Process: cellular protein metabolic process; interleukin-3-mediated signaling pathway; interleukin-5-mediated signaling pathway; MAPK cascade; peptidyl-tyrosine phosphorylation; regulation of molecular function; respiratory gaseous exchange; response to lipopolysaccharide; signal transduction

Disease: Surfactant Metabolism Dysfunction, Pulmonary, 5

Research Articles on CSF2Rb

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Product Notes

The CSF2Rb csf2rb (Catalog #AAA2002598) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CSF2Rb csf2rb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF - PPEPRDLQ ISTDQDHFLL TWSVALGSPQ SHWLSPGDLE FEVVYKRLQD SWEDAAILLS NTSQATLGPE HLMPSSTYVA RVRTRLAPGS RLSGRPSKWS PEV. It is sometimes possible for the material contained within the vial of "Colony Stimulating Factor 2 Receptor Beta (CSF2Rb), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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