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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Rat Colony Stimulating Factor 2 Polyclonal Antibody | anti-GMCSF antibody

Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

Gene Names
Csf2; Gmcsf; Gm-csf
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Colony Stimulating Factor 2; Polyclonal Antibody; Polyclonal Antibody to Colony Stimulating Factor 2; Granulocyte Macrophage (GMCSF); anti-GMCSF antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GMCSF. It has beenselected for its ability to recognize GMCSF in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-GMCSF antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
GMCSF (Ala18~Lys144)
Organism Species
Rattus norvegicus (Rat)
Cross Reactivity
Rat
Conjugate
No Conjugate
Immunogen
Recombinant GMCSF (Ala18~Lys144) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2035879
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Spleen tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded Spleen tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,562 Da
NCBI Official Full Name
granulocyte-macrophage colony-stimulating factor
NCBI Official Synonym Full Names
colony stimulating factor 2
NCBI Official Symbol
Csf2
NCBI Official Synonym Symbols
Gmcsf; Gm-csf
NCBI Protein Information
granulocyte-macrophage colony-stimulating factor
UniProt Protein Name
Granulocyte-macrophage colony-stimulating factor
UniProt Gene Name
Csf2
UniProt Synonym Gene Names
Csfgm; GM-CSF; CSF

NCBI Description

plays a role in alveolar epithelial fluid transport [RGD, Feb 2006]

Uniprot Description

GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: extracellular space; intracellular membrane-bound organelle

Molecular Function: cytokine activity; granulocyte macrophage colony-stimulating factor receptor binding; growth factor activity

Biological Process: embryonic placenta development; epithelial cell proliferation; epithelial fluid transport; histamine secretion; immune response; macrophage activation; monocyte differentiation; myeloid dendritic cell differentiation; negative regulation of cytolysis; neutrophil differentiation; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of interleukin-23 production; positive regulation of macrophage differentiation; positive regulation of tyrosine phosphorylation of STAT protein; regulation of cell proliferation; regulation of circadian sleep/wake cycle, sleep; regulation of gene expression; response to silicon dioxide

Research Articles on GMCSF

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Product Notes

The GMCSF csf2 (Catalog #AAA2027328) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Colony Stimulating Factor 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the GMCSF csf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Colony Stimulating Factor 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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