Rabbit anti-Mouse Collagen Type IV Polyclonal Antibody | anti-COL4A1 antibody

Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Base

Cat. Num. AAA6469813

• Gene Names: COL4A4; CA44
• Reactivity: Mouse
• Applications: Immunohistochemistry, immunoassay, Radioimmunoassay
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Collagen Type IV; Polyclonal Antibody; Collagen Type IV (Arresten; ASLN; ATS; CA54; Canstatin; COL4A1; COL4A2; Collagen Alpha 1(IV) Chain; Collagen Alpha 2(IV) Chain; Collagen IV Alpha 1 Polypeptide; Collagen IV Alpha 2 Polypeptide; Collagen of Basement Membrane Alpha 1 Chain; Collagen of Base; anti-COL4A1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Mouse Collagen Type IV: 100%, Mouse Collagen Types I, II, III: < 0.1%, Mouse Fibronectin: < 0.1%, Mouse Laminin: < 0.01%, Human Collagen Types IV, V: < 0.1%.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-COL4A1 antibody: Immunohistochemistry (IHC) Frozen/Paraffin, Radioimmunoassay (RIA), FLISA
• Application Notes: IHC-P: Acetone or methyl-carnoy fixed, paraffin-embedded tissue; IHC-F: 1:80. Frozen mouse skin and liver tissues.; Not suitable for use in Western Blot.; Applications are based on unconjugated antibody.
• Immunogen: Collagen type IV extracted and purified from mouse tumor tissues
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Collagen Type IV (C7510-50J), representative staining pattern/morphology on mouse liver. Tissue was pretreated with Citrate pH 6.0, antigen retrieval. Antibody was diluted to 1:50, IHC-Select reagents used with HRPDAB. Immunoreactivity is as expected since it is staining the reticulin fibers in hepatocytes)

Product Categories/Family for anti-COL4A1 antibody

Antibodies; Collagen

NCBI and Uniprot Product Information

• NCBI GI #: 116256356
• NCBI GeneID: 1286
• NCBI Accession #: NP_000083.3
• NCBI GenBank Nucleotide #: NM_000092.4
• UniProt Accession #: P53420; Q53RW9; Q53S42; Q53WR1; A8MTZ1
• Molecular Weight: 164,038 Da
• NCBI Official Full Name: collagen alpha-4(IV) chain
• NCBI Official Synonym Full Names: collagen, type IV, alpha 4
• NCBI Official Symbol: COL4A4
• NCBI Official Synonym Symbols: CA44
• NCBI Protein Information: collagen alpha-4(IV) chain; collagen alpha-4(IV) chain; Collagen IV, alpha-4 polypeptide; collagen of basement membrane, alpha-4 chain
• UniProt Protein Name: Collagen alpha-4(IV) chain
• Protein Family: Collagen
• UniProt Gene Name: COL4A4
• UniProt Entry Name: CO4A4_HUMAN

NCBI Description

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR. [provided by RefSeq, Jul 2008]

Uniprot Description

COL4A4: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A4 are a cause of Alport syndrome autosomal recessive (APSAR). APSAR is characterized by progressive glomerulonephritis, glomerular basement membrane defects, renal failure, sensorineural deafness and specific eye abnormalities (lenticonous and macular flecks). The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness. Defects in COL4A4 are a cause of benign familial hematuria (BFH); also known as thin basement membrane nephropathy. BFH is characterized by persistent hematuria, an electron microscopically detectable thin glomerular basement membrane (GBM) and an autosomal dominant mode of inheritance. Renal function remains normal. In children, differentiation between BFH and AS can be difficult, because both disorders are manifested by persistent hematuria and thin GBM at that age. Belongs to the type IV collagen family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2q35-q37

Cellular Component: endoplasmic reticulum lumen; collagen type IV; extracellular region; basal lamina

Molecular Function: extracellular matrix structural constituent

Biological Process: axon guidance; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; glomerular basement membrane development

Disease: Alport Syndrome, Autosomal Recessive