Collagen, Mouse Type IV Polyclonal Antibody
Mouse Type IV Collagen-Solution
Purity
Purity: >90% as judged by SDS-PAGE analysis and ELISAPurification: Controlled and limited pepsin digestion, followed by selective salt precipitation.
Note: This product is subjected to purification procedures involving solubility in various buffers
Synonyms
Collagen; Mouse Type IV; Polyclonal Antibody; Mouse Type IV Collagen-Solution; anti-Collagen; Mouse Type IV antibody
Host
Mouse EHS Sarcoma
Clonality
Polyclonal
Purity/Purification
Purity: >90% as judged by SDS-PAGE analysis and ELISA
Purification: Controlled and limited pepsin digestion, followed by selective salt precipitation.
Note: This product is subjected to purification procedures involving solubility in various buffers
Purification: Controlled and limited pepsin digestion, followed by selective salt precipitation.
Note: This product is subjected to purification procedures involving solubility in various buffers
Form/Format
Purified protein
Application Notes
Research Application(s): Type IV collagen standard
Antigen for antibody production
Coating material for cell culture studies
Antigen for antibody production
Coating material for cell culture studies
Characterization
To insure lot-to-lot consistency, each batch of product is tested to conform to characteristics of a standard reference reagent using SDS-PAGE analysis.
Preparation and Storage
The purified protein is supplied as a solution of 0.5 mg collagen in 1 mL of 500 mM acetic acid (C = 0.5 mg/mL).
The collagen may be transferred into physiological buffers by dialysis at 2-8 degree C.
Purified collagen may be stored at 2-8 degree C for several months without appreciable loss of activity. For long-term storage, the purified collagen may be aliquoted and stored at -20 degree C. Avoid repeated freezing and thawing.
The collagen may be transferred into physiological buffers by dialysis at 2-8 degree C.
Purified collagen may be stored at 2-8 degree C for several months without appreciable loss of activity. For long-term storage, the purified collagen may be aliquoted and stored at -20 degree C. Avoid repeated freezing and thawing.
References
1. Miller, E.J. and R.K. Rhodes. 1982. Meth. Enzymol. 82:33.
2. Miller, E.J. In: Extracellular Matrix Biochemistry, K.A. Piez and A.H. Reddi, eds. Elsevier, New York, 1984. pp. 41-81.
2. Miller, E.J. In: Extracellular Matrix Biochemistry, K.A. Piez and A.H. Reddi, eds. Elsevier, New York, 1984. pp. 41-81.
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Product Notes
The Collagen, Mouse Type IV (Catalog #AAA674537) is an Antibody produced from Mouse EHS Sarcoma and is intended for research purposes only. The product is available for immediate purchase. Research Application(s): Type IV collagen standard Antigen for antibody production Coating material for cell culture studies. Researchers should empirically determine the suitability of the Collagen, Mouse Type IV for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen, Mouse Type IV, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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