Rabbit anti-Human COLLAGEN II Polyclonal Antibody | anti-COL2A1 antibody
RABBIT ANTI HUMAN COLLAGEN II
Gene Names
COL2A1; AOM; ANFH; SEDC; COL11A3
Reactivity
Human
Applications
Immunohistochemistry, ELISA
Synonyms
COLLAGEN II; Polyclonal Antibody; RABBIT ANTI HUMAN COLLAGEN II; anti-COL2A1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Polyclonal IgG
Specificity
COLLAGEN II
This item recognizes human Collagen type II, an extracellular matrix protein found mainly in cartilaginous tissue. The antibody reacts with both native and heat denatured forms and shows minimal cross reactivity with Collagen type I, III, IV and V, as demonstrated by competitive ELISA.
This item recognizes human Collagen type II, an extracellular matrix protein found mainly in cartilaginous tissue. The antibody reacts with both native and heat denatured forms and shows minimal cross reactivity with Collagen type I, III, IV and V, as demonstrated by competitive ELISA.
Form/Format
Purified (Purified IgG - lyophilised)
Concentration
IgG concentration 0.1 mg/ml after reconstitution (varies by lot)
Sequence Length
1487
Applicable Applications for anti-COL2A1 antibody
Immunohistology - Frozen (IHC), ELISA (EIA)
Application Notes
Immunohistology - Frozen: Min Dilution: 1/10
Max Dilution: 1/50
ELISA: Min Dilution: 1/500
Max Dilution: 1/2000
Max Dilution: 1/50
ELISA: Min Dilution: 1/500
Max Dilution: 1/2000
Immunogen
Human Collagen type II from cartilage
Preparation
Purified IgG prepared by affinity chromatography on immobilized human Collagen type II
Preservative Stabilizers
1% Dextran
1% Mannitol
1% Mannitol
Reconstitution
Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide and 1% BSA is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide and 1% BSA is recommended.
Buffer Solution
Borate buffered saline
Preparation and Storage
Prior to reconstitution store at +4 degree C.
After reconstitution store at -20 degree C.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life: 12 months from date of reconstitution.
After reconstitution store at -20 degree C.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life: 12 months from date of reconstitution.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
141,785 Da
NCBI Official Full Name
collagen alpha-1(II) chain isoform 1
NCBI Official Synonym Full Names
collagen, type II, alpha 1
NCBI Official Symbol
COL2A1
NCBI Official Synonym Symbols
AOM; ANFH; SEDC; COL11A3
NCBI Protein Information
collagen alpha-1(II) chain; collagen alpha-1(II) chain; chondrocalcin; cartilage collagen; alpha-1 type II collagen; collagen II, alpha-1 polypeptide
UniProt Protein Name
Collagen alpha-1(II) chain
UniProt Gene Name
COL2A1
UniProt Entry Name
CO2A1_HUMAN
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Product Notes
The COL2A1 col2a1 (Catalog #AAA220756) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI HUMAN COLLAGEN II reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COLLAGEN II can be used in a range of immunoassay formats including, but not limited to, Immunohistology - Frozen (IHC), ELISA (EIA). Immunohistology - Frozen: Min Dilution: 1/10 Max Dilution: 1/50 ELISA: Min Dilution: 1/500 Max Dilution: 1/2000. Researchers should empirically determine the suitability of the COL2A1 col2a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COLLAGEN II, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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