Rabbit anti-Human COL8A2 Polyclonal Antibody | anti-COL8A2 antibody

COL8A2, CT (COL8A2, Collagen alpha-2(VIII) chain, Endothelial collagen) (APC)

Cat. Num. AAA6287758

• Gene Names: COL8A2; FECD; PPCD; FECD1; PPCD2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: COL8A2; Polyclonal Antibody; CT (COL8A2; Collagen alpha-2(VIII) chain; Endothelial collagen) (APC); Endothelial collagen; anti-COL8A2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human COL8A2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-COL8A2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 571-599 in the C-terminal region of human COL8A2, UniProt Accession: #P25067.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS641554. This demonstrates that MBS641554 detected the COL8A2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS641554 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS641554 for immunohistochemistry.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HepG2 cells usingMBS641554. Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) was used to visualize the antigen. DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-COL8A2 antibody

Antibodies; Collagen

References

1. Hemadevi, B., et al. BMC Ophthalmol 10, 3 (2010). 2. Afshari, N.A., et al. Invest. Ophthalmol. Vis. Sci. 50(3):1093-1097(2009). 3. Liskova, P., et al. Br J Ophthalmol 91(12):1717-1718(2007). 4. Aldave, A.J., et al. Cornea 26(8):963-965(2007). 5. Zhang, C., et al. Trans Am Ophthalmol Soc 104, 85-97 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 32964830
• NCBI GeneID: 1296
• NCBI Accession #: NP_005193.1
• NCBI GenBank Nucleotide #: NM_005202.2
• UniProt Accession #: P25067; Q5JV31; Q8TEJ5
• Molecular Weight: 67,244 Da
• NCBI Official Full Name: collagen alpha-2(VIII) chain
• NCBI Official Synonym Full Names: collagen, type VIII, alpha 2
• NCBI Official Symbol: COL8A2
• NCBI Official Synonym Symbols: FECD; PPCD; FECD1; PPCD2
• NCBI Protein Information: collagen alpha-2(VIII) chain; collagen alpha-2(VIII) chain; endothelial collagen; collagen type VIII alpha 2; collagen VIII, alpha-2 polypeptide; dJ665N4.1 (collagen type VIII alpha 2)
• UniProt Protein Name: Collagen alpha-2(VIII) chain
• UniProt Gene Name: COL8A2
• UniProt Entry Name: CO8A2_HUMAN

NCBI Description

This gene encodes the alpha 2 chain of type VIII collagen. The protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2. [provided by RefSeq, Dec 2009]

Uniprot Description

COL8A2: Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis. Defects in COL8A2 are the cause of corneal dystrophy Fuchs endothelial type 1 (FECD1). It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition. Defects in COL8A2 are the cause of posterior polymorphous corneal dystrophy type 2 (PPCD2). PPCD is a rare bilateral familial disorder of the corneal epithelium, and is inherited in a autosomal dominant pattern. The clinical features usually present earlier than FECD, being from birth onwards. The disorder is characterized by alterations of Descemet membrane presenting as vesicles, opacities or band-like lesions on slit- lamp examination and specular microscopy. Affected patient typically are asymptomatic.

Protein type: Extracellular matrix; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; collagen; endoplasmic reticulum lumen; extracellular region; basement membrane

Molecular Function: protein binding, bridging; extracellular matrix structural constituent

Biological Process: collagen catabolic process; extracellular matrix disassembly; epithelial cell proliferation; cell-cell adhesion; extracellular matrix organization and biogenesis; camera-type eye morphogenesis; angiogenesis

Disease: Corneal Dystrophy, Posterior Polymorphous, 2; Corneal Dystrophy, Fuchs Endothelial, 1