Western Blot (WB)
(Fig.1. Western Blot analysis of various cells using COL2A1 Polyclonal Antibody diluted at 1:1000.)
Rabbit COL2A1 Polyclonal Antibody | anti-COL2A1 antibody
COL2A1 Polyclonal Antibody
IF: 1:200-1:1000
IHC-P: 1:100-1:300
ELISA: 1:20000
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Western Blot (WB)
(Fig.1. Western Blot analysis of various cells using COL2A1 Polyclonal Antibody diluted at 1:1000.)
Western Blot (WB)
(Fig.1. Western Blot analysis of various cells using COL2A1 Polyclonal Antibody diluted at 1:1000.)
Immunofluorescence (IF)
(Fig.2. Immunofluorescence analysis of rat lung tissue. 1, COL2A1 Polyclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunofluorescence (IF)
(Fig.2. Immunofluorescence analysis of rat lung tissue. 1, COL2A1 Polyclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunohistochemistry (IHC)
(Fig.3. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, COL2A1 Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Fig.3. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, COL2A1 Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Fig.4. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, COL2A1 Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Fig.4. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, COL2A1 Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Fig.5. Immunohistochemical analysis of paraffin-embedded rat testis tissue. 1, COL2A1 Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Fig.5. Immunohistochemical analysis of paraffin-embedded rat testis tissue. 1, COL2A1 Polyclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
COL2A1: the alpha-1 chain of type II collagen, an extra-cellular matrix protein found in cartilage and the vitreous humor of the eye. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Chondrocalcin is the calcium binding C-propeptide of this collagen molecule. Defects in this protein are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. There are two transcripts identified for this gene. Belongs to the fibrillar collagen family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Extracellular matrix; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 12q13.11
Cellular Component: extracellular matrix; extracellular space; collagen type II; endoplasmic reticulum lumen; extracellular region; basement membrane
Molecular Function: identical protein binding; metal ion binding; platelet-derived growth factor binding; extracellular matrix structural constituent conferring tensile strength
Biological Process: heart morphogenesis; axon guidance; proteoglycan metabolic process; extracellular matrix organization and biogenesis; inner ear morphogenesis; collagen fibril organization; central nervous system development; palate development; notochord development; limb bud formation; collagen catabolic process; extracellular matrix disassembly; sensory perception of sound; visual perception; regulation of gene expression; cartilage development; tissue homeostasis; chondrocyte differentiation; skeletal development; endochondral ossification; cartilage condensation
Disease: Achondrogenesis, Type Ii; Kniest Dysplasia; Spondyloperipheral Dysplasia; Legg-calve-perthes Disease; Epiphyseal Dysplasia, Multiple, With Myopia And Conductive Deafness; Osteoarthritis With Mild Chondrodysplasia; Stickler Syndrome, Type I; Czech Dysplasia; Platyspondylic Lethal Skeletal Dysplasia, Torrance Type; Otospondylomegaepiphyseal Dysplasia; Spondyloepimetaphyseal Dysplasia, Strudwick Type; Avascular Necrosis Of Femoral Head, Primary; Spondyloepiphyseal Dysplasia Congenita; Stickler Syndrome, Type I, Nonsyndromic Ocular
Research Articles on COL2A1
Similar Products
Product Notes
The COL2A1 col2a1 (Catalog #AAA9700667) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The COL2A1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's COL2A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). WB: 1:500-1:2000 IF: 1:200-1:1000 IHC-P: 1:100-1:300 ELISA: 1:20000. Researchers should empirically determine the suitability of the COL2A1 col2a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COL2A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
