Rabbit anti-Human COL10A1 Polyclonal Antibody | anti-COL10A1 antibody

COL10A1, NT (COL10A1, Collagen alpha-1(X) chain) (Azide free) (HRP)

Cat. Num. AAA6287563

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: COL10A1; Polyclonal Antibody; NT (COL10A1; Collagen alpha-1(X) chain) (Azide free) (HRP); Collagen alpha-1(X) chain; anti-COL10A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-COL10A1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: COL10A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-58 amino acids from the N-terminal region of human COL10A1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(COL10A1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the COL10A1 antibody detected the COL10A1 protein (arrow).)

Related Product Information for anti-COL10A1 antibody

This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD).

Product Categories/Family for anti-COL10A1 antibody

Antibodies; Collagen

References

Makitie, O., et al. J. Orthop. Res. 28(11):1497-1501(2010). Wang, S., et al. Connect. Tissue Res. 51(3):188-196(2010). Videman, T., et al. Arthritis Rheum. 60(2):470-481(2009). Higashikawa, A., et al. Arthritis Rheum. 60(1):166-178(2009). Zimmermann, P., et al. Arthritis Rheum. 58(9):2743-2753(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 18105032
• NCBI GeneID: 1300
• NCBI Accession #: NP_000484.2
• NCBI GenBank Nucleotide #: NM_000493.3
• UniProt Accession #: Q03692; A1L4P2
• Molecular Weight: 66158 MW
• NCBI Official Full Name: collagen alpha-1(X) chain
• NCBI Official Synonym Full Names: collagen type X alpha 1 chain
• NCBI Official Symbol: COL10A1
• NCBI Protein Information: collagen alpha-1(X) chain
• UniProt Protein Name: Collagen alpha-1(X) chain
• Protein Family: Collagen
• UniProt Gene Name: COL10A1
• UniProt Entry Name: COAA1_HUMAN

NCBI Description

This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD). [provided by RefSeq, Jul 2008]

Uniprot Description

COL10A1: Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage. Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD). SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6q21-q22

Cellular Component: proteinaceous extracellular matrix; collagen; endoplasmic reticulum lumen; extracellular region; cell cortex

Molecular Function: metal ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; cartilage development; skeletal development; endochondral ossification

Disease: Metaphyseal Chondrodysplasia, Schmid Type