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Western Blot (WB) (Western Blot (WB) analysis of BST1 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Raw264. 7 cell lysateLane3:H9C2 cell lysate)

Rabbit BST1 Polyclonal Antibody | anti-BST1 antibody

BST1 Polyclonal Antibody

Gene Names
BST1; CD157
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
BST1; Polyclonal Antibody; BST1 Polyclonal Antibody; ADP-ribosyl cyclase 2; Bone marrow stromal antigen 1; Cyclic ADP-ribose hydrolase 2; cADPr hydrolase 2; CD157; BST-1; anti-BST1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
BST1 polyclonal antibody detects endogenous levels of BST1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
318
Applicable Applications for anti-BST1 antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
WB: 1:500-1:1000
ICC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 120-165 of Human BST1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of BST1 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Raw264. 7 cell lysateLane3:H9C2 cell lysate)

Western Blot (WB) (Western Blot (WB) analysis of BST1 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Raw264. 7 cell lysateLane3:H9C2 cell lysate)
Related Product Information for anti-BST1 antibody
BST-1 (bone marow stromal antigen-1) has been identified as a surface molecule that is GPI-anchored to the cell membrane of stromal cells. Both ADP-ribosyl cyclase and cADPR hydrolase activites have been demonstrated by BST-1. cADPR activity is a potential regulator of insulin secretion in pancreatic beta cells. Most pancreatic islet cells express BST-1, indicating a link between BST-1 and insulin secretion. BST-1 expression has also been found in a wide range of tissues including umbilical vein endothelial cells, monocytes and granulocytes. BST-1 expression in thymus tissue and on B and T cell progenitors undergoing gene rearrangement implicates BST-1 as a useful marker for lymphoid progenitor cells that are initiating gene rearrangement of their antigen receptors. BST-1 has also been shown to facilitate B cell growth and may act as a receptor.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
683
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 36kDa
NCBI Official Full Name
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
NCBI Official Synonym Full Names
bone marrow stromal cell antigen 1
NCBI Official Symbol
BST1
NCBI Official Synonym Symbols
CD157
NCBI Protein Information
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
UniProt Protein Name
ADP-ribosyl cyclase 2
Protein Family
UniProt Gene Name
BST1
UniProt Synonym Gene Names
BST-1
UniProt Entry Name
BST1_HUMAN

NCBI Description

Bone marrow stromal cell antigen-1 is a stromal cell line-derived glycosylphosphatidylinositol-anchored molecule that facilitates pre-B-cell growth. The deduced amino acid sequence exhibits 33% similarity with CD38. BST1 expression is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis. The polyclonal B-cell abnormalities in rheumatoid arthritis may be, at least in part, attributed to BST1 overexpression in the stromal cell population. [provided by RefSeq, Jul 2008]

Uniprot Description

BST1: Synthesizes cyclic ADP-ribose, a second messenger that elicits calcium release from intracellular stores. May be involved in pre-B-cell growth. Belongs to the ADP-ribosyl cyclase family.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Hydrolase; EC 3.2.2.6; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 4p15

Cellular Component: extrinsic to membrane; plasma membrane

Molecular Function: transferase activity; phosphorus-oxygen lyase activity; NAD+ nucleosidase activity; NAD(P)+ nucleosidase activity

Biological Process: metabolic process; multicellular organismal development; positive regulation of B cell proliferation; humoral immune response

Research Articles on BST1

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Product Notes

The BST1 bst1 (Catalog #AAA3005717) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BST1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BST1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). WB: 1:500-1:1000 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the BST1 bst1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BST1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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