Rabbit COH1 Polyclonal Antibody | anti-VPS13B antibody

COH1 Antibody

Cat. Num. AAA151550

• Gene Names: VPS13B; CHS1; COH1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: COH1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: COH1; Polyclonal Antibody; COH1 Antibody; CHS1; KIAA0532; Vacuolar protein sorting-associated protein 13B; Cohen syndrome protein 1; vacuolar protein sorting 13 homolog B (yeast); anti-VPS13B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least five alternatively spliced transcript variants have been observed. COH1 detects two isoforms.
• Purity/Purification: COH1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 863

• Applicable Applications for anti-VPS13B antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: COH1 antibody can be used for detection of COH1 by Western blot at 1 - 2 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: COH1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human COH1.
• Buffer: COH1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: COH1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of COH1 in SK-N-SH cell lysate with COH1 antibody at (A) 1 and (B) 2 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of COH1 in human brain tissue with COH1 antibody at 20 μg/mL.)

Related Product Information for anti-VPS13B antibody

COH1 Antibody: COH1 (Cohen syndrome protein 1), also known as VPS13B (vacuolar protein sorting-associated protein 13B) or CHS1, belongs to the VPS13 family and may function in vesicle-mediated transport and sorting of proteins within the cell. COH1 is widely expressed and multiple alternatively spliced transcript variants have been observed. Mutations in this gene have been associated with Cohen syndrome. COH1 is a Golgi-localized peripheral membrane protein and plays a critical role in Golgi (re)assembly.

NCBI and Uniprot Product Information

• NCBI GI #: 35493725
• NCBI GeneID: 157680
• NCBI Accession #: NP_056058
• NCBI GenBank Nucleotide #: NM_015243.2
• UniProt Accession #: Q7Z7G8; Q709C6; Q709C7; Q7Z7G4; Q7Z7G5; Q7Z7G6; Q7Z7G7; Q8NB77; Q9NWV1; Q9Y4E7; C9JD30
• Molecular Weight: 448,536 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 13B isoform 3
• NCBI Official Synonym Full Names: vacuolar protein sorting 13 homolog B (yeast)
• NCBI Official Symbol: VPS13B
• NCBI Official Synonym Symbols: CHS1; COH1
• NCBI Protein Information: vacuolar protein sorting-associated protein 13B
• UniProt Protein Name: Vacuolar protein sorting-associated protein 13B
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS13B
• UniProt Synonym Gene Names: CHS1; COH1; KIAA0532
• UniProt Entry Name: VP13B_HUMAN

NCBI Description

This gene encodes a potential transmembrane protein that may function in vesicle-mediated transport and sorting of proteins within the cell. This protein may play a role in the development and the function of the eye, hematological system, and central nervous system. Mutations in this gene have been associated with Cohen syndrome. Multiple splice variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

VPS13B: May be involved in protein sorting in post Golgi membrane traffic. Defects in VPS13B are a cause of Cohen syndrome (COH1). COH1 is a rare autosomal recessive disorder characterized by obesity, hypotonia, intellectual deficit, characteristic craniofacial dysmorphism and abnormalities of the hands and feet. Characteristic facial features include high-arched or wave-shaped eyelids, a short philtrum, thick hair and low hairline. Belongs to the VPS13 family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Vesicle

Chromosomal Location of Human Ortholog: 8q22.2

Biological Process: protein transport

Disease: Cohen Syndrome