Rabbit cofilin1 Polyclonal Antibody | anti-CFL1 antibody

cofilin1 (phospho-Tyr139) Antibody

Cat. Num. AAA9414745

• Gene Names: CFL1; CFL; cofilin; HEL-S-15
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: cofilin1; Polyclonal Antibody; cofilin1 (phospho-Tyr139) Antibody; CFL; CFL1; anti-CFL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of cofilin1 only when phosphorylated at tyrosine 139.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 166

• Applicable Applications for anti-CFL1 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of tyrosine 139 (N-C-Y(p)-E-E) derived from Human cofilin.
• Modification: Phospho-Tyr139
• Target Name: cofilin1
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using cofilin1(Phospho-Tyr139) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Related Product Information for anti-CFL1 antibody

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Product Categories/Family for anti-CFL1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 5031635
• NCBI GeneID: 1072
• NCBI Accession #: NP_005498.1
• NCBI GenBank Nucleotide #: NM_005507.2
• UniProt Accession #: P23528; Q53Y87; Q9UCA2; B3KUQ1
• Molecular Weight: 18,502 Da
• NCBI Official Full Name: cofilin-1
• NCBI Official Synonym Full Names: cofilin 1 (non-muscle)
• NCBI Official Symbol: CFL1
• NCBI Official Synonym Symbols: CFL; cofilin; HEL-S-15
• NCBI Protein Information: cofilin-1
• UniProt Protein Name: Cofilin-1
• Protein Family: Cofilin
• UniProt Gene Name: CFL1
• UniProt Synonym Gene Names: CFL; p18
• UniProt Entry Name: COF1_HUMAN

NCBI Description

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Uniprot Description

Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; cortical actin cytoskeleton; focal adhesion; nuclear matrix; membrane; cytoplasm; intercellular junction; nucleus; vesicle

Molecular Function: protein binding; actin binding

Biological Process: actin filament depolymerization; axon guidance; platelet activation; positive regulation of actin filament depolymerization; response to virus; cytoskeleton organization and biogenesis; negative regulation of cell size; response to amino acid stimulus; protein amino acid phosphorylation; Rho protein signal transduction; neural fold formation; platelet degranulation; cytokinesis after mitosis; protein import into nucleus; establishment of cell polarity; ephrin receptor signaling pathway; innate immune response; regulation of cell morphogenesis; actin cytoskeleton organization and biogenesis; blood coagulation; neural crest cell migration; negative regulation of apoptosis