Western Blot (WB)
(Western blot analysis of extracts from COLO205 cells using cofilin antibody and Cofilin antibody (Ser3) (Lanes 3 and 4))
Rabbit Cofilin Polyclonal Antibody | anti-CFL antibody
Cofilin Antibody (Ser3)
Gene Names
CFL1; CFL; cofilin; HEL-S-15
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Chromatography
Synonyms
Cofilin; Polyclonal Antibody; Cofilin Antibody (Ser3); Rabbit Polyclonal Cofilin Antibody (Ser3); Polyclonal Cofilin antibody; Anti-Cofilin antibody; CFL antibody; CFL1 antibody; COF1 antibody; cofilin 1 (non-muscle) antibody; Cofilin non-muscle isoform antibody; Cofilin-1 antibody; p18 antibody; anti-CFL antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Cofilin antibody (Ser3) detects endogenous levels of Cofilin only when phosphorylated at serine 3
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
166
Applicable Applications for anti-CFL antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
Cofilin antibody was raised in Rabbit using a synthesized phosphopeptide derived from human Cofilin around the phosphorylation site of serine 3 (M-A-SP-G-V) as the immunogen.
Cross-Reactivity
Mouse, Rat
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts from COLO205 cells using cofilin antibody and Cofilin antibody (Ser3) (Lanes 3 and 4))
Western Blot (WB)
(Western blot analysis of extracts from COLO205 cells using cofilin antibody and Cofilin antibody (Ser3) (Lanes 3 and 4))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cofilin antibody (Ser3))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cofilin antibody (Ser3))
Related Product Information for anti-CFL antibody
Cofilin is a ubiquitous actin-binding factor required for the reorganization of actin filaments. ADF/Cofilin family members bind G-actin monomers and depolymerize actin filaments through two mechanisms: severing and increasing the off-rate for actin monomers from the pointed end.
Product Categories/Family for anti-CFL antibody
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
Research Articles on CFL
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Product Notes
The CFL (Catalog #AAA5313580) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cofilin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the CFL for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cofilin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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