Rabbit CMPK2 Polyclonal Antibody | anti-CMPK2 antibody

CMPK2 Antibody

Cat. Num. AAA153401

• Gene Names: CMPK2; TYKi; TMPK2; UMP-CMPK2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: CMPK2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: CMPK2; Polyclonal Antibody; CMPK2 Antibody; TYKi; TMPK2; UMP-CMPK2; UMP-CMP kinase 2; mitochondrial; Nucleoside-diphosphate kinase; cytidine monophosphate (UMP-CMP) kinase 2; anti-CMPK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Multiple isoforms of CMPK2 are known to exist.
• Purity/Purification: CMPK2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 449

• Applicable Applications for anti-CMPK2 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: CMPK2 antibody can be used for detection of CMPK2 by Western blot at 1 ug/mL. For immunofluorescence start at 20 ug/mL.; Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.; ; Optimal dilutions for each application to be determined by the researcher.
• Conjugate: Unconjugated
• Immunogen: CMPK2 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human CMPK2.
• Buffer: CMPK2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CMPK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(CMPK2 Antibody)

Immunofluorescence (IF)

(Immunofluorescence of CMPK2 in human lung tissue with CMPK2 antibody at 20 μg/mL.)

Related Product Information for anti-CMPK2 antibody

CMPK2 Antibody: UMP-CMP kinase 2 (CMPK2) is the first nucleoside monophosphate kinase that has been identified in human mitochondria. It is a component of the salvage pathway for nucleotide synthesis that may participate in terminal differentiation of monocytic cells. There are two distinct domains of CMPK2: the N-terminal domain with unknown function and the C-terminal domain with the kinase function, suggesting that CMPK2 may be a bifunctional protein with other biological functions in addition to its UMP-CMP kinase activity. CMPK2 may participate in dUTP and dCTP synthesis and is responsible for phosphorylation of dCMP and dUMP in mitochondria. CMPK2 is actively involved in macrophage activation and the inflammatory response

NCBI and Uniprot Product Information

• NCBI GI #: 117606370
• NCBI GeneID: 129607
• NCBI Accession #: NP_997198
• NCBI GenBank Nucleotide #: NM_207315.3
• UniProt Accession #: Q5EBM0; Q6ZRU2; Q96AL8; A2RUB0; A5D8T2; B7ZM18
• Molecular Weight: 49 kDa
• NCBI Official Full Name: UMP-CMP kinase 2, mitochondrial isoform 1
• NCBI Official Synonym Full Names: cytidine monophosphate (UMP-CMP) kinase 2, mitochondrial
• NCBI Official Symbol: CMPK2
• NCBI Official Synonym Symbols: TYKi; TMPK2; UMP-CMPK2
• NCBI Protein Information: UMP-CMP kinase 2, mitochondrial; cytidylate kinase 2; mitochondrial UMP-CMP kinase; nucleoside-diphosphate kinase; thymidine monophosphate kinase 2; thymidylate kinase family LPS-inducible member
• UniProt Protein Name: UMP-CMP kinase 2, mitochondrial
• UniProt Gene Name: CMPK2
• UniProt Entry Name: CMPK2_HUMAN

NCBI Description

This gene encodes one of the enzymes in the nucleotide synthesis salvage pathway that may participate in terminal differentiation of monocytic cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

CMPK2: May participate in dUTP and dCTP synthesis in mitochondria. Is able to phosphorylate dUMP, dCMP, CMP, UMP and monophosphates of the pyrimidine nucleoside analogs ddC, dFdC, araC, BVDU and FdUrd with ATP as phosphate donor. Efficacy is highest for dUMP followed by dCMP; CMP and UMP are poor substrates. May be involved in mtDNA depletion caused by long term treatment with ddC or other pyrimidine analogs. Belongs to the thymidylate kinase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.4.6; Nucleotide Metabolism - pyrimidine; EC 2.7.4.14; Kinase, other

Chromosomal Location of Human Ortholog: 2p25.2

Cellular Component: nucleoplasm; mitochondrion; cytosol

Molecular Function: UMP kinase activity; nucleoside diphosphate kinase activity; thymidylate kinase activity; cytidylate kinase activity; ATP binding

Biological Process: dUDP biosynthetic process; nucleoside triphosphate biosynthetic process; dTDP biosynthetic process; dTTP biosynthetic process; nucleoside diphosphate phosphorylation