Typical Testing Data/Standard Curve
Monkey Leucine-rich alpha-2 glycoprotein 1 ELISA Kit | LRG1 elisa kit
Monkey Leucine-rich alpha-2 glycoprotein 1 ELISA Kit
Gene Names
LRG1; LRG; HMFT1766
Reactivity
Monkey
Synonyms
Leucine-rich alpha-2 glycoprotein 1; Monkey Leucine-rich alpha-2 glycoprotein 1 ELISA Kit; LRG1 elisa kit
Reactivity
Monkey
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for LRG1 elisa kit
Intended Uses: This LRG1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey LRG1. This ELISA kit for research use only!
Principle of the Assay||LRG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-LRG1 antibody and an LRG1-HRP conjugate. The assay sample and buffer are incubated together with LRG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LRG1 concentration since LRG1 from samples and LRG1-HRP conjugate compete for the anti-LRG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by LRG1 from the sample, fewer sites are left to bind LRG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LRG1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay||LRG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-LRG1 antibody and an LRG1-HRP conjugate. The assay sample and buffer are incubated together with LRG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LRG1 concentration since LRG1 from samples and LRG1-HRP conjugate compete for the anti-LRG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by LRG1 from the sample, fewer sites are left to bind LRG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LRG1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,178 Da
NCBI Official Full Name
leucine-rich alpha-2-glycoprotein
NCBI Official Synonym Full Names
leucine-rich alpha-2-glycoprotein 1
NCBI Official Symbol
LRG1
NCBI Official Synonym Symbols
LRG; HMFT1766
NCBI Protein Information
leucine-rich alpha-2-glycoprotein; 1300008B03Rik; 2310031E04Rik; leucine rich alpha 2 glycoprotein
UniProt Protein Name
Leucine-rich alpha-2-glycoprotein
Protein Family
UniProt Gene Name
LRG1
UniProt Synonym Gene Names
LRG; LRG
UniProt Entry Name
A2GL_HUMAN
NCBI Description
The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation (O'Donnell et al., 2002 [PubMed 12223515]).[supplied by OMIM, Mar 2008]
Uniprot Description
LRG1:
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: extracellular space; membrane; extracellular region
Molecular Function: transforming growth factor beta receptor binding
Biological Process: positive regulation of angiogenesis; positive regulation of transforming growth factor beta receptor signaling pathway; brown fat cell differentiation; positive regulation of endothelial cell proliferation
Research Articles on LRG1
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Product Notes
The Monkey LRG1 lrg1 (Catalog #AAA748440) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA748440 ELISA Kit recognizes Monkey LRG1. It is sometimes possible for the material contained within the vial of "Leucine-rich alpha-2 glycoprotein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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