Rabbit anti-Pig Cluster Of Differentiation 3d (CD3d) Polyclonal Antibody | anti-CD3d antibody

Polyclonal Antibody to Cluster Of Differentiation 3d (CD3d)

Cat. Num. AAA2003874

• Reactivity: Pig
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Cluster Of Differentiation 3d (CD3d); Polyclonal Antibody; Polyclonal Antibody to Cluster Of Differentiation 3d (CD3d); anti-CD3d antibody

• Host: Rabbit
• Reactivity: Pig
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against CD3d. It has been selected for its ability to recognize CD3d in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF- YEVEMEELE DKVFVSCNTS IIWLQGTEGE LLSDKKIDLG KRILDPRGLY KCNAPKEQDS NSKIFLQVYY RMCQNCVELD SAT

• Applicable Applications for anti-CD3d antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant CD3d (Tyr22~Thr103) expressed in E.coli.
• Cross Reactivity: Pig
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2060073
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Porcine Spleen Tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 47523294
• NCBI GeneID: 396661
• NCBI Accession #: NP_998940.1
• NCBI GenBank Nucleotide #: NM_213775.2
• UniProt Accession #: Q764N2
• Molecular Weight: 19,475 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 delta chain
• NCBI Official Symbol: CD3D
• NCBI Protein Information: T-cell surface glycoprotein CD3 delta chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 delta chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD3D

Uniprot Description

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.