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Western Blot (WB) (Western Blot: Sample: Human Serum; Primary Ab: 2ug/mL Rabbit Anti-Mouse CD109 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Mouse Cluster Of Differentiation 109 Polyclonal Antibody | anti-CD109 antibody

Polyclonal Antibody to Cluster Of Differentiation 109 (CD109)

Gene Names
Cd109; GARP; AI480638; 9930012E15Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Cluster Of Differentiation 109; Polyclonal Antibody; Polyclonal Antibody to Cluster Of Differentiation 109 (CD109); anti-CD109 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CD109. It has beenselected for its ability to recognize CD109 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- T VIGSERVQIT AIGDILGSSI NGLSSLIRMP YGCGEQNMIY FAPNIYILDY LTKQKQLTVN LKEKALSYMR QGYQRELLYQ REDGSFSAFG DIDSSGSTWL SAFVLRCFLE ADYYIDIDQD VLHRTYTWLN AHKKFNGEFW EPGRVIHSEL QGGTKSPVTL TAYIVTSVLG YKKYQPNIDV QDSIKFLEFE FSRGISDNYT
Applicable Applications for anti-CD109 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
CD109 (Thr890~Pro1131)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant CD109 (Thr890~Pro1131) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2055396
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Human Serum; Primary Ab: 2ug/mL Rabbit Anti-Mouse CD109 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Human Serum; Primary Ab: 2ug/mL Rabbit Anti-Mouse CD109 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Vas Deferens Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Vas Deferens Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
161,659 Da
NCBI Official Full Name
CD109 antigen
NCBI Official Synonym Full Names
CD109 antigen
NCBI Official Symbol
Cd109
NCBI Official Synonym Symbols
GARP; AI480638; 9930012E15Rik
NCBI Protein Information
CD109 antigen
UniProt Protein Name
CD109 antigen
Protein Family
UniProt Gene Name
Cd109

Uniprot Description

CD109: Modulates negatively TGFB1 signaling in keratinocytes. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 9|9 E1

Cellular Component: cell surface; extracellular region; extracellular space; membrane; plasma membrane

Molecular Function: endopeptidase inhibitor activity; peptidase inhibitor activity; serine-type endopeptidase inhibitor activity; transforming growth factor beta binding

Biological Process: hair follicle development; negative regulation of peptidase activity; negative regulation of protein phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; regulation of keratinocyte differentiation

Research Articles on CD109

Similar Products

Product Notes

The CD109 cd109 (Catalog #AAA2025998) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cluster Of Differentiation 109 (CD109) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cluster Of Differentiation 109 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the CD109 cd109 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- T VIGSERVQIT AIGDILGSSI NGLSSLIRMP YGCGEQNMIY FAPNIYILDY LTKQKQLTVN LKEKALSYMR QGYQRELLYQ REDGSFSAFG DIDSSGSTWL SAFVLRCFLE ADYYIDIDQD VLHRTYTWLN AHKKFNGEFW EPGRVIHSEL QGGTKSPVTL TAYIVTSVLG YKKYQPNIDV QDSIKFLEFE FSRGISDNYT. It is sometimes possible for the material contained within the vial of "Cluster Of Differentiation 109, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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