Rabbit anti-Human, Mouse CLSTN1 Polyclonal Antibody | anti-CLSTN1 antibody

CLSTN1 Antibody

Cat. Num. AAA9608535

• Gene Names: CLSTN1; CST-1; CSTN1; CDHR12; PIK3CD; ALC-ALPHA; XB31alpha; alcalpha1; alcalpha2
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: CLSTN1; Polyclonal Antibody; CLSTN1 Antibody; Alc alpha; Alc-alpha; Alcadein alpha 1; Alcadein alpha; Alcadein-alpha; alcalpha1; alcalpha2; Alzheimer related cadherin like protein; Alzheimer-related cadherin-like protein; C-terminal fragment 1-alpha; Cadherin related family member 12; Calsyntenin 1; Calsyntenin1; CDHR12; CLSTN 1; Clstn1; CS1; CSTN1; CSTN1_HUMAN; CTF1-alpha; FLJ32258; KIAA0911; Non classical cadherin XB31alpha; Non classical cadherin XB31alpha1; Non-classical cadherin XB31alpha; PIK3CD; SAlc-alpha; XB31alpha; anti-CLSTN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CLSTN1 antibody detects endogenous levels of total CLSTN1
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 981

• Applicable Applications for anti-CLSTN1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IHC: 1:200
• Immunogen: A synthesized peptide derived from human CLSTN1
• Subcellular Location: Endoplasmic Reticulum Membrane. Golgi Apparatus Membrane. Cell Projection. Cell Junction > Synapse > Postsynaptic Cell Membrane. Nucleus. Neurite Tips. Localized in the postsynaptic membrane of both excitatory and inhibitory synapses (By similarity). The AlcICD fragment is translocated to the nucleus upon interaction with APBB1.
• Tissue Specificity: Expressed in the brain and, a lower level, in the heart, skeletal muscle, kidney and placenta. Accumulates in dystrophic neurites around the amyloid core of Alzheimer disease senile plaques (at protein level).
• Predicted Cross Reactivity: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
• Similarity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9608535 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of CLSTN1 using LOVO whole cell lysates)

Related Product Information for anti-CLSTN1 antibody

Function: Induces KLC1 association with vesicles and functions as a cargo in axonal anterograde transport. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP. The intracellular fragment AlcICD suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding. May modulate calcium-mediated postsynaptic signals (By similarity).
Subunit Structure: Directly interacts with APBA2. Forms a tripartite complex with APBA2 and APP. The CTF1 chain interacts with PSEN1. The intracellular fragment AlcICD interacts with APBB1; this interaction stabilizes AlcICD metabolism. Interacts with KLC1 and APBB1 (By similarity).
Post-translational Modifications: Proteolytically processed under normal cellular conditions. A primary zeta-cleavage generates a large extracellular (soluble) N-terminal domain (sAlc) and a short C-terminal transmembrane fragment (CTF1). A secondary cleavage catalyzed by presenilin gamma-secretase within the transmembrane domain releases the beta-Alc-alpha chain in the extracellular milieu and produces an intracellular fragment (AlcICD). This processing is strongly suppressed in the tripartite complex formed with APBA2 and APP, which seems to prevent the association with PSEN1.
Similarity: The cytoplasmic domain is involved in interaction with APBA2, as well as the binding of synaptic Ca2+.

NCBI and Uniprot Product Information

• NCBI GI #: 57242757
• NCBI GeneID: 22883
• NCBI Accession #: NP_001009566.1
• NCBI GenBank Nucleotide #: NM_001009566.2
• UniProt Accession #: O94985; Q5SR52; Q5UE58; Q71MN0; Q8N4K9; A8K183
• Molecular Weight: Observed: 110 kDa; Predicted: 110 kDa
• NCBI Official Full Name: calsyntenin-1 isoform 1
• NCBI Official Synonym Full Names: calsyntenin 1
• NCBI Official Symbol: CLSTN1
• NCBI Official Synonym Symbols: CST-1; CSTN1; CDHR12; PIK3CD; ALC-ALPHA; XB31alpha; alcalpha1; alcalpha2
• NCBI Protein Information: calsyntenin-1
• UniProt Protein Name: Calsyntenin-1
• Protein Family: Calsyntenin
• UniProt Gene Name: CLSTN1
• UniProt Synonym Gene Names: CS1; KIAA0911; Alc-alpha; SAlc-alpha

NCBI Description

This gene is a member of the calsyntenin family, a subset of the cadherin superfamily. The encoded transmembrane protein, also known as alcadein-alpha, is thought to bind to kinesin-1 motors to mediate the axonal anterograde transport of certain types of vesicle. Amyloid precursor protein (APP) is trafficked via these vesicles and so this protein is being investigated to see how it might contribute to the mechanisms underlying Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Uniprot Description

Induces KLC1 association with vesicles and functions as a cargo in axonal anterograde transport. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP. The intracellular fragment AlcICD suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding. May modulate calcium-mediated postsynaptic signals ().