Rabbit anti-Human, Mouse CLOCK Polyclonal Antibody | anti-CLOCK antibody

CLOCK

Cat. Num. AAA616924

• Gene Names: CLOCK; KAT13D; bHLHe8; KIAA0334
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: Serum; Serum
• Synonyms: CLOCK; Polyclonal Antibody; Anti -CLOCK; anti-CLOCK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse CLOCK. Species Crossreactivity: human
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid, 0.05% sodium azide.

• Applicable Applications for anti-CLOCK antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Chromatin Immunoprecipitation.; Dilution: Western Blot: 1:1000
• Immunogen: Two peptides derived from mouse CLOCK, conjugated to albumin. Cellular Localization: Cytoplasmic and nuclear. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is BMAL1-dependent. Phosphorylated form located in the nucleus
• Cellular Localization: Cytoplasmic and nuclear. Shuffling between the cytoplasm and the nucleus is under circadian regulation and is BMAL1-dependent. Phosphorylated form located in the nucleus
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot: MBS616924 Lanes 3-5 contained alpha CLOCK immunoprecipitated DNA (15, 10 and 5ul of antisera added respectively to the 900ul of sonicated chromatin sample in the ChIP assay.)

Related Product Information for anti-CLOCK antibody

CLOCK, a basic helix-loop-helix (bHLH)/PAS protein, is an important factor in the molecular clock mechanism. Mutations in this protein lead to abnormal circadian behavior and defective transcriptional activity. CLOCK has been shown to interact strongly with other bHLH-PAS proteins such as MOP3, a BMAL1 isoform.

Product Categories/Family for anti-CLOCK antibody

Antibodies; Abs to Circadian Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 2275619
• NCBI GeneID: 9575
• UniProt Accession #: O15516; O14516; Q9UIT8; A0AV01; A2I2N9; Q3ZCT4; Q53EU0; Q8N757; Q8TAB0; Q9NYB7
• Molecular Weight: 95,304 Da
• NCBI Official Full Name: CLOCK
• NCBI Official Synonym Full Names: clock homolog (mouse)
• NCBI Official Symbol: CLOCK
• NCBI Official Synonym Symbols: KAT13D; bHLHe8; KIAA0334
• NCBI Protein Information: circadian locomoter output cycles protein kaput; OTTHUMP00000128334; OTTHUMP00000196798; OTTHUMP00000218844; class E basic helix-loop-helix protein 8; circadian locomoter output cycles kaput protein
• UniProt Protein Name: Circadian locomoter output cycles protein kaput
• Protein Family: Clock-controlled protein
• UniProt Gene Name: CLOCK
• UniProt Synonym Gene Names: BHLHE8; KIAA0334
• UniProt Entry Name: CLOCK_HUMAN

NCBI Description

This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1. [provided by RefSeq]

Uniprot Description

CLOCK: ARNTL/2-CLOCK heterodimers activate E-box element (3'- CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression. Acetylates primarily histones H3 and H4. Acetylates also a non-histone substrate: ARNTL. Plays a role in DNA damage response (DDR) signaling during the S phase. Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with ARNTL is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and ARNTL. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-ARNTL heterodimer with PER or CRY inhibits transcription activation. Binds weakly ARNTL and ARNTL2 to form heterodimers which bind poorly to the E-box motif. Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).

Protein type: DNA-binding; EC 2.3.1.48; Acetyltransferase; Transcription factor

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: nucleoplasm; transcription factor complex; intracellular membrane-bound organelle; chromosome; cytosol; nucleus

Molecular Function: protein dimerization activity; protein binding; signal transducer activity; histone acetyltransferase activity; DNA binding; sequence-specific DNA binding; chromatin DNA binding; transcription factor activity

Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; establishment and/or maintenance of chromatin architecture; positive regulation of transcription, DNA-dependent; response to redox state; signal transduction; activation of NF-kappaB transcription factor; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; photoperiodism; DNA damage checkpoint; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; histone acetylation; negative regulation of transcription, DNA-dependent; regulation of hair cycle; regulation of insulin secretion; positive regulation of inflammatory response