Rabbit CLK2 Polyclonal Antibody | anti-CLK2 antibody

CLK2 (R15) Polyclonal Antibody

Cat. Num. AAA3002029

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CLK2; Polyclonal Antibody; CLK2 (R15) Polyclonal Antibody; Dual specificity protein kinase CLK2; CDC-like kinase 2; anti-CLK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CLK2 (R15) polyclonal antibody detects endogenous levels of CLK2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 498

• Applicable Applications for anti-CLK2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human CLK2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CLK2 (R15) pAb at 1:500 dilutionLane1:C6 whole cell lysate (40ug) Lane2:3T3-L1 whole cell lysate (40ug) Lane3:HuT78 whole cell lysate (40ug) Lane4:Hela whole cell lysate (30ug) Lane5:A375 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of CLK2 (R15) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.)

Related Product Information for anti-CLK2 antibody

CLK2 (CDC-like kinase 2) is a 499 amino acid nuclear protein that contains one protein kinase domain and belongs to the Ser/Thr protein kinase family. Using ATP, CLK2 phosphorylates serine- and arginine-rich (SR) components of the spliceosomal complex, possibly playing a role in the control of RNA splicing. CLK2 exists as two alternatively spliced isoforms, designated short and long, and is encoded by a gene which maps to human chromosome 1.

NCBI and Uniprot Product Information

• NCBI GI #: 47717134
• NCBI GeneID: 1196
• NCBI Accession #: NP_003984.2
• NCBI GenBank Nucleotide #: NP_003984.2
• UniProt Accession #: P49760
• Molecular Weight: ~ 60kDa
• NCBI Official Full Name: dual specificity protein kinase CLK2 isoform 2
• NCBI Official Synonym Full Names: CDC like kinase 2
• NCBI Official Symbol: CLK2
• NCBI Protein Information: dual specificity protein kinase CLK2
• UniProt Protein Name: Dual specificity protein kinase CLK2
• Protein Family: Dual specificity protein kinase
• UniProt Gene Name: CLK2
• UniProt Entry Name: CLK2_HUMAN

NCBI Description

This gene encodes a dual specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. Activity of this protein regulates serine- and arginine-rich (SR) proteins of the spliceosomal complex, thereby influencing alternative transcript splicing. Chromosomal translocations have been characterized between this locus and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias, but encodes a protein that is involved in telomere length regulation. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Uniprot Description

CLK2: Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, dual-specificity (non-receptor); Kinase, protein; Protein kinase, CMGC; EC 2.7.12.1; CMGC group; CLK family

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; nuclear speck; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; ATP binding

Biological Process: response to retinoic acid; peptidyl-tyrosine phosphorylation; regulation of RNA splicing; protein amino acid autophosphorylation; negative regulation of gluconeogenesis; response to ionizing radiation; protein amino acid phosphorylation