Rabbit CLIP170 Polyclonal Antibody | anti-CLIP1 antibody

CLIP170 Antibody

Cat. Num. AAA150236

• Gene Names: CLIP1; RSN; CLIP; CYLN1; CLIP170; CLIP-170
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: CLIP170 Antibody is affinity chromatography purified via peptide column.
• Synonyms: CLIP170; Polyclonal Antibody; CLIP170 Antibody; RSN; CLIP; CYLN1; CLIP-170; CAP-Gly domain-containing linker protein 1; Cytoplasmic linker protein 1; CAP-GLY domain containing linker protein 1; anti-CLIP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: CLIP170 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1427

• Applicable Applications for anti-CLIP1 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: CLIP170 antibody can be used for detection of CLIP170 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunoflourescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: CLIP170 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human CLIP170.
• Buffer: CLIP170 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CLIP170 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CLIP170 in rat brain tissue lysate with CLIP170 antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunofluorescence (IF)

(Immunofuorescence of CLIP170 in human brain tissue with CLIP170 antibody at 20 μg/mL.)

Related Product Information for anti-CLIP1 antibody

CLIP170 Antibody: CLIP170 was initially identified as a new type of intermediate filament associated protein that is highly expressed in Reed-Sternberg cells, the tumoral cells for Hodgkin's disease. Later experiments showed that it is located at microtubule plus ends and is required for the binding of endocytic carrier vesicles. CLIP170 has also been suggested to act with LIS1, a protein implicated in brain development, to regulate dynein/dynactin binding microtubules. Other studies suggest that CLIP170 can influence the formation of lamellipodia and cell invasion by invasive breast cancer cells by regulating the release of kinesin and IQGAP1 from a complex of those proteins, CLIP170 and Rac1. At least two isoforms of CLIP170 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 4506751
• NCBI GeneID: 6249
• NCBI Accession #: NP_002947
• NCBI GenBank Nucleotide #: NM_002956.2
• UniProt Accession #: P30622; Q17RS4; Q29RG0; A0AVD3
• Molecular Weight: 156,781 Da
• NCBI Official Full Name: CAP-Gly domain-containing linker protein 1 isoform a
• NCBI Official Synonym Full Names: CAP-GLY domain containing linker protein 1
• NCBI Official Symbol: CLIP1
• NCBI Official Synonym Symbols: RSN; CLIP; CYLN1; CLIP170; CLIP-170
• NCBI Protein Information: CAP-Gly domain-containing linker protein 1; cytoplasmic linker protein 1; cytoplasmic linker protein CLIP-170; cytoplasmic linker protein 170 alpha-2; restin (Reed-Steinberg cell-expressed intermediate filament-associated protein)
• UniProt Protein Name: CAP-Gly domain-containing linker protein 1
• Protein Family: CAP-Gly domain-containing linker protein
• UniProt Gene Name: CLIP1
• UniProt Synonym Gene Names: CYLN1; RSN; CLIP-170
• UniProt Entry Name: CLIP1_HUMAN

NCBI Description

The protein encoded by this gene links endocytic vesicles to microtubules. This gene is highly expressed in Reed-Sternberg cells of Hodgkin disease. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

Restin: Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking. Interacts with MTOR; phosphorylates and regulates CLIP1. Interacts (via CAP-Gly domains) with tubulin. Interacts with SLAIN2. Interacts (via zinc finger) with DCTN1. Interacts with MAPRE1 and MAPRE3. Detected in dendritic cells. Highly expressed in the Reed-Sternberg cells of Hodgkin disease. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q24.3

Cellular Component: microtubule cytoskeleton; kinetochore; ruffle; centrosome; microtubule; cytoplasmic vesicle membrane; cytoplasmic microtubule; cytoplasm; intermediate filament; cytosol; endosome

Molecular Function: tubulin binding; microtubule plus-end binding; protein binding; protein homodimerization activity; nucleic acid binding; zinc ion binding; metal ion binding; microtubule binding

Biological Process: mitosis; positive regulation of microtubule polymerization; mitotic cell cycle; microtubule bundle formation