Rabbit anti-Human, Mouse CLIP1 Polyclonal Antibody | anti-CLIP1 antibody

CLIP1, NT (Cytoplasmic Linker Protein 1, Reed-Sternberg Intermediate Filament-Associated Protein, Cytoplasmic Linker Protein 170 alpha-2, CLIP-170, Restin, CAP-Gly Domain-containing Linker Protein 1, CYLN1, RSN) (Biotin)

Cat. Num. AAA6469463

• Gene Names: CLIP1; RSN; CLIP; CYLN1; CLIP170; CLIP-170
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: CLIP1; Polyclonal Antibody; NT (Cytoplasmic Linker Protein 1; Reed-Sternberg Intermediate Filament-Associated Protein; Cytoplasmic Linker Protein 170 alpha-2; CLIP-170; Restin; CAP-Gly Domain-containing Linker Protein 1; CYLN1; RSN) (Biotin); anti-CLIP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CLIP1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CLIP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human CLIP1 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CLIP1 Antibody (N-term) in Hela cell line lysates (35ug/lane). CLIP1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of CLIP1 Antibody (N-term) in mouse cerebellum tissue lysates (35ug/lane). CLIP1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(CLIP1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLIP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CLIP1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CLIP1 antibody

Antibodies; Proteins

References

1. Yang, X., et.al., J. Biol. Chem. 284(42), 28775-28782 (2009). 2. Meunier, B., et.al., Eur. J. Cell Biol. 88(2), 91-102 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4506751
• NCBI GeneID: 6249
• NCBI Accession #: NP_002947.1
• NCBI GenBank Nucleotide #: NM_002956.2
• UniProt Accession #: P30622; Q17RS4; Q29RG0; A0AVD3
• Molecular Weight: 156,781 Da
• NCBI Official Full Name: CAP-Gly domain-containing linker protein 1 isoform a
• NCBI Official Synonym Full Names: CAP-GLY domain containing linker protein 1
• NCBI Official Symbol: CLIP1
• NCBI Official Synonym Symbols: RSN; CLIP; CYLN1; CLIP170; CLIP-170
• NCBI Protein Information: CAP-Gly domain-containing linker protein 1; cytoplasmic linker protein 1; cytoplasmic linker protein CLIP-170; cytoplasmic linker protein 170 alpha-2; restin (Reed-Steinberg cell-expressed intermediate filament-associated protein)
• UniProt Protein Name: CAP-Gly domain-containing linker protein 1
• Protein Family: CAP-Gly domain-containing linker protein
• UniProt Gene Name: CLIP1
• UniProt Synonym Gene Names: CYLN1; RSN; CLIP-170
• UniProt Entry Name: CLIP1_HUMAN

NCBI Description

The protein encoded by this gene links endocytic vesicles to microtubules. This gene is highly expressed in Reed-Sternberg cells of Hodgkin disease. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

Restin: Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking. Interacts with MTOR; phosphorylates and regulates CLIP1. Interacts (via CAP-Gly domains) with tubulin. Interacts with SLAIN2. Interacts (via zinc finger) with DCTN1. Interacts with MAPRE1 and MAPRE3. Detected in dendritic cells. Highly expressed in the Reed-Sternberg cells of Hodgkin disease. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q24.3

Cellular Component: microtubule cytoskeleton; kinetochore; ruffle; centrosome; microtubule; cytoplasmic vesicle membrane; cytoplasmic microtubule; cytoplasm; intermediate filament; cytosol; endosome

Molecular Function: tubulin binding; microtubule plus-end binding; protein binding; protein homodimerization activity; nucleic acid binding; zinc ion binding; metal ion binding; microtubule binding

Biological Process: mitosis; positive regulation of microtubule polymerization; mitotic cell cycle; microtubule bundle formation