Western Blot (WB)
((1ug/ml) staining of human testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Clik1/STK35 Polyclonal Antibody | anti-STK35 antibody
Goat anti-Clik1/STK35 Antibody
Gene Names
STK35; CLIK1; STK35L1
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Clik1/STK35; Polyclonal Antibody; Goat anti-Clik1/STK35 Antibody; STK35; Clik1; bA550O8.2; bA550O8.2 CLIK1; serine/threonine kinase 35; CLP-36 interacting kinase; CLIK1; STK35L1; OTTHUMP00000030006; serine threonine kinase 35 long form; STK35 antibody; Clik1 antibody; bA550O8.2 antibody; bA550O8.2 CLIK1 antibody; serine/threonine kinase 35 antibody; CLP-36 interacting kinase antibody; CLIK1 antibody; STK35L1 antibody; OTTHUMP00000030006 antibody; serine threonine kinase 35 long form antibody; Clik1 / STK35; anti-STK35 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
ELETRMDQVTCAA
Sequence Length
534
Applicable Applications for anti-STK35 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded human testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration, 10ug/ml.
Western Blot: Approx 45kDa band observed in human testis lysates (calculated MW of 44.6kDa according to NP_543026. Recommended concentration: 1-3ug/ml.
Immunohistochemistry: In paraffin embedded human testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration, 10ug/ml.
Western Blot: Approx 45kDa band observed in human testis lysates (calculated MW of 44.6kDa according to NP_543026. Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence ELETRMDQVTCAA, from the C Terminus of the protein sequence according to NP_543026.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of human testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of human testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((10ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.)
Immunohistochemistry (IHC)
((10ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,051 Da
NCBI Official Full Name
serine/threonine-protein kinase 35
NCBI Official Synonym Full Names
serine/threonine kinase 35
NCBI Official Symbol
STK35
NCBI Official Synonym Symbols
CLIK1; STK35L1
NCBI Protein Information
serine/threonine-protein kinase 35
UniProt Protein Name
Serine/threonine-protein kinase 35
Protein Family
UniProt Gene Name
STK35
UniProt Synonym Gene Names
CLIK1; PDIK1; STK35L1; CLIK-1
UniProt Entry Name
STK35_HUMAN
NCBI Description
The protein encoded by this gene is a kinase that is predominantly found in the nucleus. However, it can interact with PDLIM1/CLP-36 in the cytoplasm and localize to actin stress fibers. The encoded protein may be a regulator of actin stress fibers in nonmuscle cells. [provided by RefSeq, Jul 2008]
Uniprot Description
CLIK1: is a kinase that is predominantly found in the nucleus. However, it can interact with PDLIM1/CLP-36 in the cytoplasm and localize to actin stress fibers. The encoded protein may be a regulator of actin stress fibers in nonmuscle cells. [provided by RefSeq, Jul 2008]
Protein type: Nucleolus; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; Other group; NKF4 family
Chromosomal Location of Human Ortholog: 20p13
Cellular Component: cytoplasm; nucleolus
Molecular Function: ATP binding; protein serine/threonine kinase activity
Biological Process: protein amino acid phosphorylation
Research Articles on STK35
Similar Products
Product Notes
The STK35 stk35 (Catalog #AAA420787) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Clik1/STK35 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Clik1/STK35 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: In paraffin embedded human testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration, 10ug/ml. Western Blot: Approx 45kDa band observed in human testis lysates (calculated MW of 44.6kDa according to NP_543026. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the STK35 stk35 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ELETRMDQVT CAA. It is sometimes possible for the material contained within the vial of "Clik1/STK35, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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