Rabbit CLIC5 Polyclonal Antibody | anti-CLIC5 antibody

CLIC5 Antibody

Cat. Num. AAA9629337

• Gene Names: CLIC5; MST130; DFNB102; DFNB103; MSTP130
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: CLIC5; Polyclonal Antibody; CLIC5 Antibody; Chloride intracellular channel 5; Chloride intracellular channel 5A; Chloride intracellular channel protein 5; CLIC 5; CLIC 5B; CLIC5_HUMAN; CLIC5B; dJ447E21.4; FLJ90663; MST 130; MST130; MSTP 130; MSTP130; anti-CLIC5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: CLIC5 Antibody detects endogenous levels of total CLIC5.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CLIC5 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion (By similarity). Belongs to the chloride channel CLIC family.
• Subunit Structure: Component of a multimeric complex consisting of several cytoskeletal proteins, including actin, ezrin, alpha-actinin, gelsolin, and IQGAP1. Interacts with AKAP9.
• Tissue Specificity: Widely expressed in both fetal and adult human tissues. Isoform 1 is expressed in renal glomeruli endothelial cells and podocytes (at protein level).
• Function: Required for normal hearing. It is necessary for the formation of stereocilia in the inner ear and normal development of the organ of Corti (By similarity). Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture. Plays a role in formation of the lens suture in the eye, which is important for normal optical properties of the lens (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-CLIC5 antibody

Cytoplasm; Cytoskeleton; Cytoplasm; Cell cortex; Membrane; Single-pass membrane protein; Apical cell membrane; Single-pass membrane protein; Primary; Microtubule organizing center; Centrosome

NCBI and Uniprot Product Information

• NCBI GI #: 82617632
• NCBI GeneID: 53405
• NCBI Accession #: NP_058625.2
• NCBI GenBank Nucleotide #: NM_016929.4
• UniProt Accession #: Q9NZA1; Q5T4Z0; Q8NBY3; Q96JT5; Q9BWZ0; B3KUF1
• Molecular Weight: 22,830 Da
• NCBI Official Full Name: chloride intracellular channel protein 5 isoform b
• NCBI Official Synonym Full Names: chloride intracellular channel 5
• NCBI Official Symbol: CLIC5
• NCBI Official Synonym Symbols: MST130; DFNB102; DFNB103; MSTP130
• NCBI Protein Information: chloride intracellular channel protein 5
• UniProt Protein Name: Chloride intracellular channel protein 5
• Protein Family: Chloride intracellular channel protein
• UniProt Gene Name: CLIC5
• UniProt Entry Name: CLIC5_HUMAN

NCBI Description

This gene encodes a member of the chloride intracellular channel (CLIC) family of chloride ion channels. The encoded protein associates with actin-based cytoskeletal structures and may play a role in multiple processes including hair cell stereocilia formation, myoblast proliferation and glomerular podocyte and endothelial cell maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

CLIC5: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Required for normal formation of stereocilia in the inner ear and normal development of the organ of Corti. Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture. Belongs to the chloride channel CLIC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: Golgi apparatus; stereocilium; microtubule organizing center; cell cortex; actin cytoskeleton

Molecular Function: protein binding; chloride channel activity; voltage-gated ion channel activity

Biological Process: diet induced thermogenesis; protein localization; sensory perception of sound; transport; chloride transport; auditory receptor cell stereocilium organization and biogenesis; female pregnancy; neuromuscular process controlling balance

Disease: Deafness, Autosomal Recessive 103